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    products/primary-antibodies/erbb4--her4-phospho-y1284-antibody-ab61059.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases
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Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (4)

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Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)
  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)

Key features and details

  • Rabbit polyclonal to ErbB4 / HER4 (phospho Y1284)
  • Suitable for: WB, ELISA, IHC-FoFr, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-ErbB4 / HER4 (phospho Y1284) antibody
    See all ErbB4 / HER4 primary antibodies
  • Description

    Rabbit polyclonal to ErbB4 / HER4 (phospho Y1284)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-FoFr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ErbB4/ HER4 aa 1250-1350 (phospho Y1284).
    Database link: Q15303

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human ErbB4 / HER4 protein (ab155610)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab61059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21903113
ICC/IF (1)
1/100 - 1/500.
Notes
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21903113
ICC/IF
1/100 - 1/500.

Target

  • Function

    Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.
  • Tissue specificity

    Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
    Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
    Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.
  • Cellular localization

    Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
  • Target information above from: UniProt accession Q15303 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2066 Human
    • Entrez Gene: 13869 Mouse
    • Entrez Gene: 59323 Rat
    • Omim: 600543 Human
    • SwissProt: Q15303 Human
    • SwissProt: Q61527 Mouse
    • SwissProt: Q62956 Rat
    • Unigene: 390729 Human
    • Unigene: 442420 Mouse
    • Unigene: 163078 Rat
    see all
  • Alternative names

    • 4ICD antibody
    • ALS19 antibody
    • Avian erythroblastic leukemia viral oncogene homolog 4 antibody
    • Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody
    • E4ICD antibody
    • EC 2.7.10.1 antibody
    • ErbB 4 antibody
    • Erbb4 antibody
    • ERBB4 intracellular domain antibody
    • ERBB4_HUMAN antibody
    • HER 4 antibody
    • HER4 antibody
    • human epidermal growth factor receptor 4 antibody
    • Mer4 antibody
    • MGC138404 antibody
    • Oncogene ERBB4 antibody
    • p180erbB4 antibody
    • Proto-oncogene-like protein c-ErbB-4 antibody
    • Receptor protein tyrosine kinase erbB 4 precursor antibody
    • Receptor tyrosine protein kinase erbB 4 antibody
    • s80HER4 antibody
    • Tyrosine kinase type cell surface receptor HER4 antibody
    • Tyrosine kinase-type cell surface receptor HER4 antibody
    • v erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody
    • v erb a erythroblastic leukemia viral oncogene homolog 4 antibody
    • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) antibody
    • V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4 antibody
    • Verba avian erythroblastic leukemia viral oncogene homolog like 4 antibody
    • Verba erythroblastic leukemia viral oncogene homolog 4 antibody
    • VERBB2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)This image is courtesy of an Abreview submitted by Simon Pascual

    ab61059 staining ErbB4 / HER4 (phospho Y1284) in rat heart tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were fixed with paraformaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% BSA + normal goat serum for 4 hours at 21°C. Samples were incubated with primary antibody (1/30 in 5% BSA + 0.05% Tween20 in TBS) for 12 hours at 4°C. ab150077, a goat anti-rabbit  Alexa 488 (1/200) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)
    Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)

    1/100-1/500 ab61059 staining phosphorilated ErbB4 / HER4 on HeLa cells treated with 200nM EGF for 5 minutes, in the absence (left) or presence (right) of the immunizing phosphopeptide.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab61059? Please let us know so that we can cite the reference in this datasheet.

ab61059 has been referenced in 4 publications.

  • Yin Y  et al. Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition. Biomed Res Int 2019:6595437 (2019). PubMed: 31317035
  • Wang J  et al. Qiliqiangxin protects against anoxic injury in cardiac microvascular endothelial cells via NRG-1/ErbB-PI3K/Akt/mTOR pathway. J Cell Mol Med 21:1905-1914 (2017). PubMed: 28271613
  • Depboylu C  et al. Systemically administered neuregulin-1ß1 rescues nigral dopaminergic neurons via the ErbB4 receptor tyrosine kinase in MPTP mouse models of Parkinson's disease. J Neurochem 133:590-7 (2015). IHC-FoFr ; Mouse . PubMed: 25581060
  • Rösler TW  et al. Biodistribution and brain permeability of the extracellular domain of neuregulin-1-ß1. Neuropharmacology 61:1413-8 (2011). IHC-FoFr ; Mouse . PubMed: 21903113

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-ErbB 4 (phospho Y1284) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA and Normal Goat Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Rat Cell (Heart sections)
Specification
Heart sections
Permeabilization
Yes - Tween20 0.05%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Simon Pascual

Verified customer

Submitted Apr 15 2014

Western blot abreview for Anti-ErbB 4 (phospho Y1284) antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary lung epithelial cells and MLE12 cells)
Loading amount
30 µg
Specification
Primary lung epithelial cells and MLE12 cells
Treatment
33nM neuregulin for 5min in primary lung epithelial cells or 0-30 min in MLE12
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 18 2010

Western blot abreview for Anti-ErbB 4 (phospho Y1284) antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 cells)
Loading amount
25 µg
Specification
MCF7 cells
Treatment
33 nm Neuregulin for 0 - 30 mins
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 18 2010

Question

Please suggest sopme ErbB4 antibodies that will detect the extracellular domain. For human samples and ICC, live and fixed cells.

Read More

Abcam community

Verified customer

Asked on Sep 02 2011

Answer

Thank you for your telephone call earlier today. According to Swissprot/Uniprot protein database site, the extracellular domain of the ErbB 4 is from amino acids 26 – 651. http://www.uniprot.org/uniprot/Q15303 We do have the following ErbB4 antibodies tested in ICC-If and human, but regrettably, their immunogen seuqences are all in the cytoplasmic domain. They have been tested in ICC on fixed cells. As discussed on the telephone, I am sorry they will not have been tested and guarnateed for live cell imaging. I am copying the details below in case they are still of interest to you. I can recommend to review the online datasheets for further information. ab19391 Anti-ErbB 4 antibody [HFR1] Mouse monoclonal Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB Reacts with: Human, Mouse Immunogen: RSTLQHPDYLQEYST amino acids 1250-1264 Click here (or use the following: https://www.abcam.com/ErbB4--HER4-antibody-HFR-1-ab19391.html). ab109273 Anti-ErbB 4 (phospho Y1284) antibody [EPR2273(2)] Rabbit monoclonal Applications: Flow Cyt, ICC/IF, WB Reacts with: Human, Mouse, Rat Immunogen: residues surrounding phosphorylated Tyrosine 1284 Click here (or use the following: https://www.abcam.com/ErbB4--HER4-phospho-Y1284-antibody-EPR22732-ab109273.html). ab61059 Anti-ErbB 4 (phospho Y1284) antibody Rabbit polyclonal Applications: ELISA, ICC/IF, WB Reacts with: Human, Mouse, Rat Immunogen: phosphorylation site of tyrosine 1284 (P-E-YP-L-S) Click here (or use the following: https://www.abcam.com/ErbB4--HER4-phospho-Y1284-antibody-ab61059.html). ab63354 Anti-ErbB 4 antibody Rabbit polyclonal Applications: ELISA, ICC/IF, IHC-P, WB Reacts with: Human Immunogen: phosphorylation site of tyrosine 1284. Click here (or use the following: https://www.abcam.com/ErbB4--HER4-antibody-ab63354.html). I am sorry we do not have antibodies that exactly meet your requirements on this occasion, but I hope this will still be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Sep 02 2011

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