Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)
Key features and details
- Rabbit polyclonal to ErbB4 / HER4 (phospho Y1284)
- Suitable for: WB, ELISA, IHC-FoFr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ErbB4 / HER4 (phospho Y1284) antibody
See all ErbB4 / HER4 primary antibodies -
Description
Rabbit polyclonal to ErbB4 / HER4 (phospho Y1284) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, IHC-FoFr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ErbB4/ HER4 aa 1250-1350 (phospho Y1284).
Database link: Q15303 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 21903113
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| ICC/IF | (1) |
1/100 - 1/500.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 21903113 |
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ICC/IF
1/100 - 1/500. |
Target
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Function
Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2. -
Tissue specificity
Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsIsoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4. -
Cellular localization
Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2066 Human
- Entrez Gene: 13869 Mouse
- Entrez Gene: 59323 Rat
- Omim: 600543 Human
- SwissProt: Q15303 Human
- SwissProt: Q61527 Mouse
- SwissProt: Q62956 Rat
- Unigene: 390729 Human
see all -
Alternative names
- 4ICD antibody
- ALS19 antibody
- Avian erythroblastic leukemia viral oncogene homolog 4 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)This image is courtesy of an Abreview submitted by Simon Pascual
ab61059 staining ErbB4 / HER4 (phospho Y1284) in rat heart tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Sections were fixed with paraformaldehyde, permeabilized with 0.05% Tween20 and blocked with 5% BSA + normal goat serum for 4 hours at 21°C. Samples were incubated with primary antibody (1/30 in 5% BSA + 0.05% Tween20 in TBS) for 12 hours at 4°C. ab150077, a goat anti-rabbit Alexa 488 (1/200) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB4 / HER4 (phospho Y1284) antibody (ab61059)1/100-1/500 ab61059 staining phosphorilated ErbB4 / HER4 on HeLa cells treated with 200nM EGF for 5 minutes, in the absence (left) or presence (right) of the immunizing phosphopeptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab61059 has been referenced in 4 publications.
- Yin Y et al. Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition. Biomed Res Int 2019:6595437 (2019). PubMed: 31317035
- Wang J et al. Qiliqiangxin protects against anoxic injury in cardiac microvascular endothelial cells via NRG-1/ErbB-PI3K/Akt/mTOR pathway. J Cell Mol Med 21:1905-1914 (2017). PubMed: 28271613
- Depboylu C et al. Systemically administered neuregulin-1ß1 rescues nigral dopaminergic neurons via the ErbB4 receptor tyrosine kinase in MPTP mouse models of Parkinson's disease. J Neurochem 133:590-7 (2015). IHC-FoFr ; Mouse . PubMed: 25581060
- Rösler TW et al. Biodistribution and brain permeability of the extracellular domain of neuregulin-1-ß1. Neuropharmacology 61:1413-8 (2011). IHC-FoFr ; Mouse . PubMed: 21903113