Recombinant Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)
![Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)](https://www.abcam.com/ps/products/109/ab109273/Images/ab109273-485123-AntiErbB4-HER4-phospho-Y1284-antibody-EPR22732-western-blot-a431-human-2.jpg "Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2273(2)] to ErbB4 / HER4 (phospho Y1284)
- Suitable for: WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)]
See all ErbB4 / HER4 primary antibodies -
Description
Rabbit monoclonal [EPR2273(2)] to ErbB4 / HER4 (phospho Y1284) -
Host species
Rabbit -
Specificity
This antibody detects ErbB4 / HER4 phosphorylated at Tyrosine 1284 and may also detect Erbb2/HER2 phosphorylated at Tyrosine 1248.
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
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Tested applications
Suitable for: WB, Dot blotmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 treated with 50mM pervanadate for 5 min whole cell lysate and A431 treated with 100 ng/ml Epidermal Growth Factor (EGF) for 30 min whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2273(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 147 kDa).
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| Dot blot |
1/1000.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 147 kDa). |
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Dot blot
1/1000. |
Application notes
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-P or IP.
Target
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Function
Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2. -
Tissue specificity
Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsIsoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4. -
Cellular localization
Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2066 Human
- Omim: 600543 Human
- SwissProt: Q15303 Human
- Unigene: 390729 Human
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Alternative names
- 4ICD antibody
- ALS19 antibody
- Avian erythroblastic leukemia viral oncogene homolog 4 antibody
see all
Images
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Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)All lanes : Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273) at 1/1000 dilution
Lane 1 : Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 treated with 50mM pervanadate for 5 min whole cell lysate
Lane 3 : A431 treated with 50mM pervanadate for 5 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)](https://www.abcam.com/ps/products/109/ab109273/Images/ab109273-485103-AntiErbB4-HER4-phospho-Y1284-antibody-EPR22732-western-blot-a431-human.jpg)
Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)All lanes : Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273) at 1/1000 dilution
Lane 1 : Untreated A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 treated with 100 ng/ml Epidermal Growth Factor (EGF) for 30 min whole cell lysate
Lane 3 : A431 treated with 100 ng/ml Epidermal Growth Factor (EGF) for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 147 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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![Dot Blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)](https://www.abcam.com/ps/products/109/ab109273/Images/ab109273-485101-AntiErbB4-HER4-phospho-Y1284-antibody-EPR22732-dot-blot.jpg)
Dot Blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)Dot blot analysis using 1/1000 dilution ab109273 and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary at 1/100000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST
Lane 1: ErbB4 non-phospho peptide
Lane 2: ErbB4 Y1284 phospho peptide
Lane 3: ErbB2 non-phospho peptide
Lane 4: ErbB2 Y1248 phospho peptide
Exposure time: 3 minutes
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![Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)](https://www.abcam.com/ps/products/109/ab109273/Images/ab109273-4-benefits-of-recombinant-antibodies.png)
Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (ab109273)
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab109273 has been referenced in 7 publications.
- Hu X et al. Sustained ErbB Activation Causes Demyelination and Hypomyelination by Driving Necroptosis of Mature Oligodendrocytes and Apoptosis of Oligodendrocyte Precursor Cells. J Neurosci 41:9872-9890 (2021). PubMed: 34725188
- Yan T et al. Multi-region sequencing unveils novel actionable targets and spatial heterogeneity in esophageal squamous cell carcinoma. Nat Commun 10:1670 (2019). PubMed: 30975989
- Liu M et al. Neuregulin-1 attenuates experimental cerebral malaria (ECM) pathogenesis by regulating ErbB4/AKT/STAT3 signaling. J Neuroinflammation 15:104 (2018). PubMed: 29636063
- Chen J et al. A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov 3:17044 (2017). PubMed: 29238610
- Shekhar A et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest 126:4444-4459 (2016). IHC-Fr ; Mouse . PubMed: 27775552
- Liang X et al. Activation of NRG1-ERBB4 signaling potentiates mesenchymal stem cell-mediated myocardial repairs following myocardial infarction. Cell Death Dis 6:e1765 (2015). PubMed: 25996292
- Slanina H et al. Role of epidermal growth factor receptor signaling in the interaction of Neisseria meningitidis with endothelial cells. Infect Immun 82:1243-55 (2014). PubMed: 24379285