Anti-ERK1 + ERK2 antibody (ab196883)
Key features and details
- Rabbit polyclonal to ERK1 + ERK2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-ERK1 + ERK2 antibody
See all ERK1 + ERK2 primary antibodies -
Description
Rabbit polyclonal to ERK1 + ERK2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human ERK 1/2 (Total) (internal sequence). The exact sequence is proprietary.
Database link: P28482 -
Positive control
- WB: K562 and NIH/3T3 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
Target
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Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Omim: 176948 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- SwissProt: P28482 Human
see all -
Form
Mainly expressed in the cytoplasm and only localizes to the nucleus with treatment. -
Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK-1 antibody
see all
Images
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Western blot - Anti-ERK1 + ERK2 antibody (ab196883)All lanes : Anti-ERK1 + ERK2 antibody (ab196883) at 1/500 dilution
Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) cell extract
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell extract with synthesized peptide
Predicted band size: 41 kDa -
Western blot - Anti-ERK1 + ERK2 antibody (ab196883)All lanes : Anti-ERK1 + ERK2 antibody (ab196883) at 1/500 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract with synthesized peptide
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extract
Predicted band size: 41 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (43)
ab196883 has been referenced in 43 publications.
- Liang X & Ju J Matrine inhibits ovarian cancer cell viability and promotes apoptosis by regulating the ERK/JNK signaling pathway via p38MAPK. Oncol Rep 45:N/A (2021). PubMed: 33786627
- Wang L et al. N2E4, a Monoclonal Antibody Targeting Neuropilin-2, Inhibits Tumor Growth and Metastasis in Pancreatic Ductal Adenocarcinoma via Suppressing FAK/Erk/HIF-1a Signaling. Front Oncol 11:657008 (2021). PubMed: 34336654
- Zhang D et al. Dexamethasone and lenvatinib inhibit migration and invasion of non-small cell lung cancer by regulating EKR/AKT and VEGF signal pathways. Exp Ther Med 19:762-770 (2020). PubMed: 31853327
- Zhang H et al. Overexpression of HIF-1a protects PC12 cells against OGD/R-evoked injury by reducing miR-134 expression. Cell Cycle 19:990-999 (2020). PubMed: 32266863
- Fan L et al. Sevoflurane inhibits the migration and invasion of colorectal cancer cells through regulating ERK/MMP-9 pathway by up-regulating miR-203. Eur J Pharmacol 850:43-52 (2019). PubMed: 30685432