Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab214362)
Key features and details
- Rabbit polyclonal to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody -
Description
Rabbit polyclonal to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Synthetic peptide within Human ERK1 aa 150-250 (phospho T202 + Y204) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P27361 -
Positive control
- Human colon carcinoma lysate; Human liver tissue and lung carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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| WB |
1/100 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 41 kDa).
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| Notes |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
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WB
1/100 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 41 kDa). |
Target
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Cellular localization
ERK2: Nucleus. -
Database links
- Entrez Gene: 327672 Cow
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
see all
Images
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Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab214362)Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab214362) at 1/200 dilution + Human colon carcinoma lysate
Secondary
Goat anti-rabbit IgG (H+L), HRP at 1/3000 dilution
Predicted band size: 41 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?12% Gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab214362)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling ERK 1/2 (phospho-Thr202/Tyr204) with ab214362 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab214362)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling ERK 1/2 (phospho-Thr202/Tyr204) with ab214362 at 1/200 dilution followed by Goat anti-rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C . Nuclei are stained with DAPI.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (130)
ab214362 has been referenced in 130 publications.
- Hu X et al. Bone marrow mesenchymal stem cell-derived exosomal miR-34c-5p ameliorates RIF by inhibiting the core fucosylation of multiple proteins. Mol Ther 30:763-781 (2022). PubMed: 34678513
- Wu Y et al. Knockdown of CCL28 inhibits endometriosis stromal cell proliferation and invasion via ERK signaling pathway inactivation. Mol Med Rep 25:N/A (2022). PubMed: 34913072
- Liao H et al. B7‑H3 promotes the epithelial‑mesenchymal transition of NSCLC by targeting SIRT1 through the PI3K/AKT pathway. Mol Med Rep 25:N/A (2022). PubMed: 35029291
- Song Z et al. Baicalin Attenuated Aβ1-42-Induced Apoptosis in SH-SY5Y Cells by Inhibiting the Ras-ERK Signaling Pathway. Biomed Res Int 2022:9491755 (2022). PubMed: 35528169
- Zhang DD et al. Geniposide Alleviates Neuropathic Pain in CCI Rats by Inhibiting the EGFR/PI3K/AKT Pathway And Ca2+ Channels. Neurotox Res 40:1057-1069 (2022). PubMed: 35699893