Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)
![Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-283000-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-western-blot.jpg "Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)")
Key features and details
- Rabbit monoclonal [SP327] to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] -
Description
Rabbit monoclonal [SP327] to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 + EGF cell lysates. IHC-P: Human breast ductal carcinoma, bladder transitional cell carcinoma and breast tissues. Flow Cyt: A431 + EGF cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SP327 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
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| WB |
1/400. Predicted molecular weight: 42,44 kDa.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/100. |
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WB
1/400. Predicted molecular weight: 42,44 kDa. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Cellular localization
ERK2: Nucleus. -
Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Omim: 176948 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- SwissProt: P28482 Human
- Unigene: 431850 Human
- Unigene: 861 Human
Images
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Western blot - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)All lanes : Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500) at 1/400 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) + EGF cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Predicted band size: 42,44 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-282997-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)Paraffin-embedded human breast ductal carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
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![Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-282999-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)Flow cytometric analysis of ab223500 in A431 (human epidermoid carcinoma cell line) cells + EGF (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-282996-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)Paraffin-embedded human breast tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)](https://www.abcam.com/ps/products/223/ab223500/Images/ab223500-282995-anti-erk1-erk2-phospho-t202-y204-antibody-sp327-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] (ab223500)Paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (46)
ab223500 has been referenced in 46 publications.
- Du X et al. ITGB4 deficiency induces mucus hypersecretion by upregulating MUC5AC in RSV-infected airway epithelial cells. Int J Biol Sci 18:349-359 (2022). PubMed: 34975337
- Zhou J et al. The ILEI/LIFR complex induces EMT via the Akt and ERK pathways in renal interstitial fibrosis. J Transl Med 20:54 (2022). PubMed: 35093095
- Zhuang Q et al. Knockdown of circ-RAD23B inhibits non-small cell lung cancer progression via the miR-142-3p/MAP4K3 axis. Thorac Cancer 13:750-760 (2022). PubMed: 35106926
- Yu Y et al. Targeting Forkhead box O1-aquaporin 5 axis mitigates neuropathic pain in a CCI rat model through inhibiting astrocytic and microglial activation. Bioengineered 13:8567-8580 (2022). PubMed: 35324416
- Liu S et al. Inhibitory effect of lovastatin on human lung cancer cell proliferation by regulating the ERK1/2 and COX-2 pathways. Transl Cancer Res 11:813-822 (2022). PubMed: 35571660