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RecombinantRabMAb

Recombinant Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP327] to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt (Intra), WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free

  • Description

    Rabbit monoclonal [SP327] to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 + EGF cell lysates. IHC-P: Human breast ductal carcinoma, bladder transitional cell carcinoma and breast tissues. Flow Cyt (intra): A431 + EGF cells.

  • General notes

    ab242418 is the carrier-free version of ab223500.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab242418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.

Target

Images

  • Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)
    Flow Cytometry (Intracellular) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)

    Intracellular flow cytometric analysis of ab223500 in A431 (human epidermoid carcinoma cell line) cells + EGF (green) at 1/100 dilution compared to negative control of rabbit IgG (blue). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223500). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)

    Paraffin-embedded human breast ductal carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)

    Paraffin-embedded human breast tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [SP327] - BSA and Azide free (ab242418)

    Paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERK1 + ERK2 (phospho T202 + Y204) using ab223500 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab223500).

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab242418? Please let us know so that we can cite the reference in this datasheet.

ab242418 has been referenced in 1 publication.

  • Mao FY  et al. Helicobacter pylori-Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense. Cell Mol Gastroenterol Hepatol 12:395-425 (2021). PubMed: 33676046

Customer reviews and Q&As

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