Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)
Key features and details
- Rabbit polyclonal to ERK1 (phospho Y204) + ERK2 (phospho Y187)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody -
Description
Rabbit polyclonal to ERK1 (phospho Y204) + ERK2 (phospho Y187) -
Host species
Rabbit -
Specificity
This antibody is specific for ERK1, only when phosphorylated at tyrosine 204 -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human ERK1 (phospho Y204).
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Positive control
- Extracts from Jurkat cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 44, 42 kDa).
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
1/500 - 1/1000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 44, 42 kDa). |
Target
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Cellular localization
ERK2: Nucleus. -
Database links
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- Entrez Gene: 116590 Rat
- Entrez Gene: 50689 Rat
- Omim: 176948 Human
- Omim: 601795 Human
see all -
Alternative names
- ERK 1 antibody
- ERK 2 antibody
- ERK antibody
see all
Images
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Western blot - Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)All lanes : Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells treated with PMA (200ng/ml, 15min)
Lane 2 : Extracts from Jurkat cells un-treated
Predicted band size: 44, 42 kDa
Observed band size: 42,44 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)Ab47339 staining human normal or colon tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Western blot - Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)All lanes : Anti-ERK1 (phospho Y204) + ERK2 (phospho Y187) antibody (ab47339)
Lane 1 : Jurkat cell extract
Lane 2 : Jurkat cell extract treated with PMA
Predicted band size: 44, 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab47339 has been referenced in 25 publications.
- Chang HX & Zhao YF Protocatechuic acid as an inhibitor of the JNK/CXCL1/CXCR2 pathway relieves neuropathic pain in CCI rats. Bosn J Basic Med Sci 22:217-228 (2022). PubMed: 34813418
- Choucair N et al. Immunohistochemical assessment of cannabinoid type-1 receptor (CB1R) and its correlation with clinicopathological parameters in glioma. Pathologica 114:128-137 (2022). PubMed: 35481563
- Han N et al. The G-Protein-Coupled Estrogen Receptor (GPER) Regulates Trimethylation of Histone H3 at Lysine 4 and Represses Migration and Proliferation of Ovarian Cancer Cells In Vitro. Cells 10:N/A (2021). PubMed: 33799631
- Ye Y et al. Prostaglandin E2 receptor 3 (EP3) signaling promotes migration of cervical cancer via urokinase-type plasminogen activator receptor (uPAR). J Cancer Res Clin Oncol 146:2189-2203 (2020). PubMed: 32488496
- Zhang Q et al. G6PD facilitates clear cell renal cell carcinoma invasion by enhancing MMP2 expression through ROS-MAPK axis pathway. Int J Oncol 57:197-212 (2020). PubMed: 32319593