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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ERp29 antibody [EPR12499(B)] (ab176573)

Submit a review Submit a question References (4)
Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
  • Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
  • Anti-ERp29 antibody [EPR12499(B)] (ab176573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12499(B)] to ERp29
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ERp29 antibody [EPR12499(B)]
    See all ERp29 primary antibodies

  • Description

    Rabbit monoclonal [EPR12499(B)] to ERp29

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow

  • Immunogen

    Synthetic peptide within Human ERp29 aa 50-150 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: P30040

  • Positive control

    • WB: HEK293T, HeLa, HepG2, MCF7 and OVCAR-3 cell lysates. ICC/IF: HeLa cells. IHC-P: Human kidney and liver tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12499(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
ICC/IF
1/100 - 1/250.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

  • Tissue specificity

    Ubiquitous. Mostly expressed in secretory tissues.

  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C12orf8 antibody
    • Chromosome 12 open reading frame 8 antibody
    • Endoplasmic reticulum lumenal protein ERp28 antibody
    • Endoplasmic reticulum protein 29 antibody
    • Endoplasmic reticulum protein 29 isoform 1 antibody
    • Endoplasmic reticulum protein ERp29 antibody
    • Endoplasmic reticulum resident protein 28 antibody
    • Endoplasmic reticulum resident protein 29 antibody
    • Endoplasmic reticulum resident protein 31 antibody
    • Epididymis secretory protein Li 107 antibody
    • ERp28 antibody
    • ERp29 antibody
    • ERP29_HUMAN antibody
    • ERp31 antibody
    • HEL S 107 antibody
    • PDI-DB antibody
    • PDIA9 antibody
    • Protein disulfide isomerase family A member 9 antibody
    see all

Images

  • Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    All lanes : Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ERP29 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab176573 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab176573 Anti-ERp29 antibody [EPR12499(B)] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266543 (knockout cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. ab176573 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    All lanes : Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : OVCAR-3 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 using ab176573 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ERp29 using ab176573 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12499(B)] (ab176573)

    Immunofluorescent analysis of HeLa cells, labeling ERp29 using ab176573 at a 1/00 dilution (red). DAPI nuclear staining (blue).

  • Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Anti-ERp29 antibody [EPR12499(B)] (ab176573)

References (4)

Publishing research using ab176573? Please let us know so that we can cite the reference in this datasheet.

ab176573 has been referenced in 4 publications.

  • Liu F  et al. Dexmedetomidine alleviates insulin resistance in hepatocytes by reducing endoplasmic reticulum stress. Endocrine 67:87-94 (2020). PubMed: 31679138
  • Carron J  et al. Role of a genetic variation in the microRNA-4421 binding site of ERP29 regarding risk of oropharynx cancer and prognosis. Sci Rep 10:17039 (2020). PubMed: 33046743
  • Ye W  et al. ERp29 downregulation enhances lung adenocarcinoma cell chemosensitivity to gemcitabine by upregulating HSP27 phosphorylation. Exp Ther Med 17:817-823 (2019). PubMed: 30651868
  • Chaiyawat P  et al. Endoplasmic reticulum protein 29 (ERp29) as a novel prognostic marker and tumor suppressor in osteosarcoma. J Bone Oncol 16:100233 (2019). PubMed: 30984555

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