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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-ERp29 antibody [EPR12985] (ab175193)

Reviews (1) Submit a question
Western blot - Anti-ERp29 antibody [EPR12985] (ab175193)
  • Western blot - Anti-ERp29 antibody [EPR12985] (ab175193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12985] (ab175193)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12985] (ab175193)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12985] (ab175193)
  • Anti-ERp29 antibody [EPR12985] (ab175193)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12985] to ERp29
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-ERp29 antibody [EPR12985]
    See all ERp29 primary antibodies

  • Description

    Rabbit monoclonal [EPR12985] to ERp29

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, MCF7, A431, and HEK293T cell lysate. IHC-P: Human kidney and thyroid gland tissues. ICC/IF: MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12985

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF
1/50 - 1/100.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.

  • Tissue specificity

    Ubiquitous. Mostly expressed in secretory tissues.

  • Cellular localization

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    • Alternative names

      • C12orf8 antibody
      • Chromosome 12 open reading frame 8 antibody
      • Endoplasmic reticulum lumenal protein ERp28 antibody
      • Endoplasmic reticulum protein 29 antibody
      • Endoplasmic reticulum protein 29 isoform 1 antibody
      • Endoplasmic reticulum protein ERp29 antibody
      • Endoplasmic reticulum resident protein 28 antibody
      • Endoplasmic reticulum resident protein 29 antibody
      • Endoplasmic reticulum resident protein 31 antibody
      • Epididymis secretory protein Li 107 antibody
      • ERp28 antibody
      • ERp29 antibody
      • ERP29_HUMAN antibody
      • ERp31 antibody
      • HEL S 107 antibody
      • PDI-DB antibody
      • PDIA9 antibody
      • Protein disulfide isomerase family A member 9 antibody
      see all

    Images

    • Western blot - Anti-ERp29 antibody [EPR12985] (ab175193)
      Western blot - Anti-ERp29 antibody [EPR12985] (ab175193)
      All lanes : Anti-ERp29 antibody [EPR12985] (ab175193) at 1/500 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : ERP29 knockout HEK293T cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 29 kDa
      Observed band size: 29 kDa



      Lanes 1-3: Merged signal (red and green). Green - ab175193 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.

      ab175193 Anti-ERp29 antibody [EPR12985] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266543 (knockout cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. ab175193 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-ERp29 antibody [EPR12985] (ab175193)
      Western blot - Anti-ERp29 antibody [EPR12985] (ab175193)
      All lanes : Anti-ERp29 antibody [EPR12985] (ab175193) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12985] (ab175193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12985] (ab175193)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 with ab175193 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12985] (ab175193)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12985] (ab175193)

      Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling ERp29 with ab175193 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12985] (ab175193)
      Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12985] (ab175193)

      Immunofluorescence analysis of MCF7 cells labeling ERp29 with ab175193 at a 1/50 dilution (red) and DAPI nuclear staining (blue).

    • Anti-ERp29 antibody [EPR12985] (ab175193)
      Anti-ERp29 antibody [EPR12985] (ab175193)

    References (0)

    Publishing research using ab175193? Please let us know so that we can cite the reference in this datasheet.

    ab175193 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-ERp29 antibody [EPR12985]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (lung)
    Gel Running Conditions
    Reduced Denaturing (4-15)
    Loading amount
    20 µg
    Specification
    lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 01 2017

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