Anti-ERp57 antibody (ab10287)
Key features and details
- Rabbit polyclonal to ERp57
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ERp57 antibody
See all ERp57 primary antibodies -
Description
Rabbit polyclonal to ERp57 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Mouse ERp57.
Database link: P27773 -
Positive control
- WB: Mouse embryonic cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000 - 1/3000. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa).
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/1000 - 1/3000. Detects a band of approximately 57 kDa (predicted molecular weight: 61 kDa). |
Target
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Tissue specificity
Detected in the flagellum and head region of spermatozoa (at protein level). -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 2923 Human
- Entrez Gene: 14827 Mouse
- Omim: 602046 Human
- SwissProt: P30101 Human
- SwissProt: P27773 Mouse
- Unigene: 591095 Human
- Unigene: 263177 Mouse
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Alternative names
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
see all
Images
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Western blot - Anti-ERp57 antibody (ab10287)All lanes : Anti-ERp57 antibody (ab10287) at 1/1000 dilution
Lane 1 : Mouse embryonic cells
Lane 2 : Calreticulin deficient mouse embryomic cells
Predicted band size: 61 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?Western blot using ab10287 at 1/1000.
Lane 1 – Mouse Embryonic Fibroblasts.
Lane 2 – Calreticulin deficient Mouse Embryonic Fibroblasts. -
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)Double staining of mouse embryonic fibroblasts (top) and calreticulin deficient fibroblasts (bottom) with rabbit-anti-ERp57 antibodies (1:100; upper and lower left panel) and FITC-ConA (upper and lower right panel). -
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab10287)This image is courtesy of an anonymous Abreviewab10287 at 1/100 staining human HeLa cells by ICC/IF. The cells were premeabilized with digitonin before being blocked and then stained with the antibody for 1 hour. An Alexa-Fluor ® conjugated goat anti rabbit antibody was used as the secondary.
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Western blot - Anti-ERp57 antibody (ab10287)Anti-ERp57 antibody (ab10287) at 1/1000 dilution +Recombinant Human ERp57 protein (ab92937) at 0.01 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Exposure time: 150 seconds
Protocols
Datasheets and documents
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Datasheet download
References (37)
ab10287 has been referenced in 37 publications.
- Mao Z et al. KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge. Cell Discov 8:5 (2022). PubMed: 35075146
- Domnick A et al. Molecular basis of MHC I quality control in the peptide loading complex. Nat Commun 13:4701 (2022). PubMed: 35948544
- Kasahara J et al. Proteomic analysis of subcellular compartments containing disseminated alpha-synuclein seeds. Neurosci Res 170:341-349 (2021). PubMed: 33309865
- Liang YH et al. Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing. Sci Rep 11:9080 (2021). PubMed: 33907276
- Ocklenburg T et al. In oxygen-deprived tumor cells ERp57 provides radioprotection and ensures proliferation via c-Myc, PLK1 and the AKT pathway. Sci Rep 11:7199 (2021). PubMed: 33785835
