Recombinant Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)](https://www.abcam.com/ps/products/252/ab252794/Images/ab252794-449315-AntiEstrogen-Receptor-alpha-antibody-IHC2.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [CRET94D] to Estrogen Receptor alpha - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free
See all Estrogen Receptor alpha primary antibodies -
Description
Mouse monoclonal [CRET94D] to Estrogen Receptor alpha - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human breast cancer tissue, mouse breast tissue and mouse ovary tissue lysates. IHC: Human breast carcinoma and mouse breast tissue.
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General notes
ab252794 is the carrier-free version of ab241557.
This product has switched from a hybridoma to recombinant production method on 7th December 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CRET94D -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/1000. Predicted molecular weight: 66 kDa.
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| Notes |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000. Predicted molecular weight: 66 kDa. |
Target
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Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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Database links
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Omim: 133430 Human
- SwissProt: P03372 Human
- SwissProt: P19785 Mouse
- Unigene: 208124 Human
- Unigene: 463262 Mouse
- Unigene: 9213 Mouse
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Alternative names
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab241557).
Immunohistochemical analysis of paraffin-embedded mouse breast labeling Estrogen Receptor with ab252794 at 1/1000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab252794 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab133469, 1:5000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylinFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)](https://www.abcam.com/ps/products/252/ab252794/Images/ab252794-449314-AntiEstrogen-Receptor-alpha-antibody-IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab241557).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma and mouse breast tissues labeling Estrogen Receptor with ab252794 at 1/1000 dilution followed by LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab252794 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab133469, 1:5000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylinFor other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)](https://www.abcam.com/ps/products/252/ab252794/Images/ab252794-449313-AntiEstrogen-Receptor-alpha-antibody-Western-blot.jpg)
Western blot - Anti-Estrogen Receptor alpha antibody [CRET94D] - BSA and Azide free (ab252794)All lanes : Anti-Estrogen Receptor alpha antibody [CRET94D] (ab241557) at 1/1000 dilution
Lane 1 : Human breast cancer tissue lysates
Lane 2 : Mouse breast tissue lysates
Lane 3 : Mouse ovary tissue lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368)
Predicted band size: 66 kDa
Observed band size: 66 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab252794 has not yet been referenced specifically in any publications.
