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    products/primary-antibodies/estrogen-receptor-alpha-antibody-epr4097-ab108398.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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RecombinantRabMAb

Recombinant Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

  • Datasheet
  • SDS
Reviews (11)Q&A (1)References (25)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Flow Cytometry (Intracellular) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
  • Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4097] to Estrogen Receptor alpha
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-Estrogen Receptor alpha antibody [EPR4097]
    See all Estrogen Receptor alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR4097] to Estrogen Receptor alpha
  • Host species

    Rabbit
  • Specificity

    Expression levels of ER alpha protein vary with sample type.
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7 and T47-D cell lysates. IHC-Fr: Frozen Human cervix and uterus tissue sections. IHC-P: Human breast ductal infiltrating carcinoma and normal breast tissues. ICC/IF: MCF-7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4097
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Estrogen
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Estrogen
    • Cancer
    • Tumor biomarkers
    • Receptors

Associated products

  • Alternative Versions

    • Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (ab167610)
    • Alexa Fluor® 647 Anti-Estrogen Receptor alpha antibody [EPR4097] (ab205851)
    • Alexa Fluor® 594 Anti-Estrogen Receptor alpha antibody [EPR4097] (ab207046)
    • Alexa Fluor® 555 Anti-Estrogen Receptor alpha antibody [EPR4097] (ab279333)
  • Assay kits

    • Estrogen Receptor Transcription Factor Assay Kit (Colorimetric) (ab207203)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Estrogen Receptor alpha protein (ab82606)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB (5)
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.

For unpurified use at 1/200 - 1/1000.

IHC-P (3)
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

IHC-Fr
Use a concentration of 5 µg/ml.
ICC/IF
1/250.

For unpurified use at 1/30.

Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Predicted molecular weight: 66 kDa.

For unpurified use at 1/200 - 1/1000.

IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/30.

IHC-Fr
Use a concentration of 5 µg/ml.
ICC/IF
1/250.

For unpurified use at 1/30.

Target

  • Function

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localization

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Target information above from: UniProt accession P03372 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2099 Human
    • Omim: 133430 Human
    • SwissProt: P03372 Human
    • Unigene: 208124 Human
    • Alternative names

      • 7*/654 isoform antibody
      • 7*/819 2 isoform antibody
      • 7*/822 isoform antibody
      • 8*/901 isoform antibody
      • 8*/941 isoform antibody
      • DKFZp686N23123 antibody
      • ER alpha antibody
      • ER antibody
      • ER-alpha antibody
      • Era antibody
      • ESR antibody
      • ESR1 antibody
      • ESR1_HUMAN antibody
      • ESRA antibody
      • Estradiol receptor antibody
      • Estrogen nuclear receptor alpha antibody
      • Estrogen receptor 1 antibody
      • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
      • Estrogen receptor alpha antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
      • Estrogen receptor alpha delta 4 +49 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
      • Estrogen receptor alpha E1 E2 1 2 antibody
      • Estrogen receptor alpha E1 N2 E2 1 2 antibody
      • Estrogen receptor antibody
      • ESTRR antibody
      • NR3A1 antibody
      • Nuclear receptor subfamily 3 group A member 1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue labelling Estrogen Receptor alpha with purified ab108398 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    • Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      All lanes : Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398) at 1/2000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates
      Lane 2 : T-47D (human mammary gland ductal carcinoma epithelial cell). Whole cell lysates
      Lane 3 : MDA-MB231 (Human breast adenocarcinoma epithelial cell) Whole cell lysates (Negative control)
      Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) Whole cell lysates (Negative control)
      Lane 5 : Human uterus whole tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 66 kDa
      Observed band size: 68 kDa why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398) at 1/1000 dilution + GST-Tagged Recombinant Human Estrogen Receptor beta (aa 1 to 323) protein 100ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 66 kDa


      Exposure time: 180 seconds


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      Lane 1: Recombinant Human Estrogen Receptor beta (aa 1 to 323) protein, (Cat#: ab158385)
      There is no cross-reactivity between ab108398 and ER beta protein.

    • Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Western blot - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      All lanes : purified at 1/2000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : T47-D cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 66 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      Immunocytochemsitry/Immunofluorescence analysis of MCF-7 cells labelling Estrogen Receptor alpha (green) with purified ab108398 at 1/200. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

    • Flow Cytometry (Intracellular) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Flow Cytometry (Intracellular) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      ab108398 staining Estrogen Receptor alphain the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody. 

      Isoytype control: Rabbit monoclonal IgG (Black) 

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast ductal infiltrating carcinoma tissue labelling Estrogen Receptor alpha with unpurified ab108398.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human normal tonsil tissue. Unpurified ab108398 shows negative staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human colonic adenocarcinoma tissue. Unpurified ab108398 shows negative staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human lung adenocarcinoma tissue. Unpurified ab108398 shows negative staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human cervical carcinoma tissue. Unpurified ab108398 shows negative staining.

    • Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      IHC image of Estrogen Receptor alpha staining in a section of frozen human uterus* performed on a Leica Biosystems BOND® RX instrumen using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab108398, 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      IHC image of Estrogen Receptor alpha staining in a section of frozen human cervix* performed on a Leica Biosystems BOND® RX instrumen using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab108398, 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

      Negative control image: IHC image of Estrogen Receptor alpha staining in a section of frozen human hippocampus* performed on a Leica Biosystems BOND® RX instrumen using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab108398, 5 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

    • Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)
      Anti-Estrogen Receptor alpha antibody [EPR4097] (ab108398)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (25)

    Publishing research using ab108398? Please let us know so that we can cite the reference in this datasheet.

    ab108398 has been referenced in 25 publications.

    • Cheng JN  et al. A Role for TGFß Signaling in Preclinical Osteolytic Estrogen Receptor-Positive Breast Cancer Bone Metastases Progression. Int J Mol Sci 22:N/A (2021). PubMed: 33923316
    • Cheng R  et al. Expression of IL17A in endometrial carcinoma and effects of IL17A on biological behaviour in Ishikawa cells. J Int Med Res 48:300060520950563 (2020). PubMed: 32878530
    • Gao Y  et al. Acetylation of histone H3K27 signals the transcriptional elongation for estrogen receptor alpha. Commun Biol 3:165 (2020). PubMed: 32265480
    • Zhang KJ  et al. miR-574-5p attenuates proliferation, migration and EMT in triple-negative breast cancer cells by targeting BCL11A and SOX2 to inhibit the SKIL/TAZ/CTGF axis. Int J Oncol 56:1240-1251 (2020). PubMed: 32319565
    • Han Y  et al. Inhibition of CDK2 reduces EZH2 phosphorylation and reactivates ERa expression in high-grade serous ovarian carcinoma. Am J Cancer Res 10:1194-1206 (2020). PubMed: 32368395
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (Breast tissue)
    Permeabilization
    Yes - PBS 1X+Triton 0.3%
    Specification
    Breast tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Emmanuel Bouchaert

    Verified customer

    Submitted Feb 12 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Breast tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1 buffer (High pH Tris based)
    Permeabilization
    No
    Specification
    Breast tissue
    Fixative
    formaline 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Emmanuel Bouchaert

    Verified customer

    Submitted Jan 24 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (breast tumor xenograft in mouse)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    breast tumor xenograft in mouse
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 24 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TE pH9
    Permeabilization
    No
    Specification
    Breast tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 24 2019

    Western blot abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Cell lysate - whole cell (Pig Mammary Epithelial Cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    15 µg
    Specification
    Pig Mammary Epithelial Cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    Western blot abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Spodoptera frugiperda (SF9 cells) Cell lysate - whole cell (sf9 cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100000 cells
    Treatment
    30 nM Bortezomib for 16 hours
    Specification
    sf9 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    Western blot abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (rat intestinal epithelial cells (RIE-1))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100000 cells
    Treatment
    100 nM Bortezomib for 16 hours
    Specification
    rat intestinal epithelial cells (RIE-1)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    Western blot abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Chinese Hamster Cell lysate - whole cell (Chinese hamster ovary cell)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100000 cells
    Treatment
    100 nM Bortezomib for 16 hours
    Specification
    Chinese hamster ovary cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 10 2016

    IHC - Wholemount abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Tissue (Invasive ductal carcinoma)
    Specification
    Invasive ductal carcinoma
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 19 2015

    Western blot abreview for Anti-Estrogen Receptor alpha antibody [EPR4097] - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (COS-7 cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    100000 cells
    Treatment
    30 nM Bortezomib for 24 hours
    Specification
    COS-7 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 26 2015

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