Recombinant Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)
![Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)](https://www.abcam.com/ps/products/32/ab32396/Images/ab32396-290776-anti-estrogen-receptor-alpha-phospho-s118-antibody-e91-immunohistochemistry.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E91] to Estrogen Receptor alpha (phospho S118)
- Suitable for: Dot blot, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Estrogen Receptor alpha (phospho S118) antibody [E91]
See all Estrogen Receptor alpha primary antibodies -
Description
Rabbit monoclonal [E91] to Estrogen Receptor alpha (phospho S118) -
Host species
Rabbit -
Specificity
ab32396 only detects ER alpha phosphorylated on Serine 118. -
Tested applications
Suitable for: Dot blot, WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Estrogen Receptor alpha. The exact sequence is proprietary.
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Positive control
- MCF7 cell lysate human breast adenocarcinoma
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E91 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot |
1/1000.
|
|
| WB | (2) |
1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
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| ICC/IF |
1/200.
For unpurified use at 1/50 - 1/100 dilution. |
| Notes |
|---|
|
Dot blot
1/1000. |
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WB
1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
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ICC/IF
1/200. For unpurified use at 1/50 - 1/100 dilution. |
Target
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Function
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsPhosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
Cellular localization
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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Database links
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
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Alternative names
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) treated with EGF (100ng/ml, 5min) and treated with Lambda Protein Phosphatase 31℃ for 2h cells labeling Estrogen receptor alpha (phospho S118) with purified ab32396 at 1:200 dilution (8.9μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Western blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)](https://www.abcam.com/ps/products/32/ab32396/Images/ab32396-273308-anti-estrogen-receptor-alpha-phospho-s118-antibody-e91-western-blot.jpg)
Western blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)All lanes : Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396) at 1/1000 dilution (purified)
Lane 1 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with epidermal growth factor. Whole cell lysates
Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) treated with epidermal growth factor. Whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)](https://www.abcam.com/ps/products/32/ab32396/Images/ab32396-273309-anti-estrogen-receptor-alpha-phospho-s118-antibody-e91-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 5 μg/ml (1/200 dilution). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077, an AlexaFluor®488 Goat anti-Rabbit was used as the secondary antibody at 2 μg/ml (1/1000 dilution). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain at 2.5 μg/ml (1/200 dilution). DAPI nuclear counterstain.
Confocal image showing the signal increased after EGF (100ng/ml, 5 min) treatment and decreased after Lambda Protein Phosphatase treatment (31°C for 2 hours). -
![Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)](https://www.abcam.com/ps/products/32/ab32396/Images/ab32396-273307-anti-estrogen-receptor-alpha-phospho-s118-antibody-e91-dot-blot.jpg)
Dot Blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)Dot blot analysis of Lane 1: Estrogen Receptor alpha (pS118) phospho peptide and Lane 2: Estrogen Receptor alpha non-phospho peptide labeling Estrogen Receptor alpha (phospho S118) with unpurified ab32396 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051, Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
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![Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)](https://www.abcam.com/ps/products/32/ab32396/Images/ab32396-44699-anti-estrogen-receptor-alpha-phospho-s118-antibody-e91-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)Immunofluorescent staining of (A) untreated and (B) Phosphatase-treated MCF-7 cells using unpurified ab32396.
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![Western blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)](https://www.abcam.com/ps/products/32/ab32396/Images/ab32396-44700-anti-estrogen-receptor-alpha-phospho-s118-antibody-e91-western-blot.jpg)
Western blot - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)All lanes : Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396) at 1/1000 dilution (unpurified)
Lane 1 : MCF7 cell lysate (untreated).
Lane 2 : MCF7 cell lysate (treated with b-Estradiol and EGF).
Predicted band size: 66 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)](https://www.abcam.com/ps/products/32/ab32396/Images/ab32396-8-benefits-of-recombinant-antibodies.png)
Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (ab32396)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab32396 has been referenced in 17 publications.
- Zhao Z et al. Crosstalk between the muscular estrogen receptor a and BDNF/TrkB signaling alleviates metabolic syndrome via 7,8-dihydroxyflavone in female mice. Mol Metab 45:101149 (2021). PubMed: 33352311
- Ohlsson C et al. Phosphorylation site S122 in estrogen receptor a has a tissue-dependent role in female mice. FASEB J 34:15991-16002 (2020). PubMed: 33067917
- Ma Y et al. Loss of Estrogen Efficacy Against Hippocampus Damage in Long-Term OVX Mice Is Related to the Reduction of Hippocampus Local Estrogen Production and Estrogen Receptor Degradation. Mol Neurobiol 57:3540-3551 (2020). PubMed: 32542593
- Castellaro AM et al. Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells. Cancers (Basel) 11:N/A (2019). PubMed: 30736340
- Huang S et al. Targeting Pin1 by All-Trans Retinoic Acid (ATRA) Overcomes Tamoxifen Resistance in Breast Cancer via Multifactorial Mechanisms. Front Cell Dev Biol 7:322 (2019). PubMed: 31867329