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    products/primary-antibodies/estrogen-receptor-beta-1-antibody-erb455-c-terminal-ab187291.pdf

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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Estrogen
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Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
  • Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
  • Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
  • Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

Key features and details

  • Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Sheep, Horse, Human, Pig, Monkey
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal
  • Description

    Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal
  • Host species

    Mouse
  • Specificity

    Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Horse, Human, Pig, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human Estrogen Receptor beta 1 (C terminal).
    Database link: Q92731

  • Positive control

    • Flow Cyt: BT474 and MCF-7 cells IHC-P: Ovarian carcinoma, breast carcinoma tissues, gastric carcinoma, and bladder carcinoma tissues. WB: MCF7 cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab187291 was purified from Bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    ERb455
  • Isotype

    IgG2a
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Estrogen
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Estrogen
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Estrogen
    • Cancer
    • Tumor biomarkers
    • Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P (6)
Use a concentration of 1 - 2 µg/ml.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Flow Cyt
Use 1-2µg for 106 cells.
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml.

Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Flow Cyt
Use 1-2µg for 106 cells.

Target

  • Relevance

    This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 100033964 Horse
    • Entrez Gene: 2100 Human
    • Entrez Gene: 13983 Mouse
    • Entrez Gene: 396697 Pig
    • Entrez Gene: 25149 Rat
    • Entrez Gene: 443066 Sheep
    • Omim: 601663 Human
    • SwissProt: Q92731 Human
    • SwissProt: O08537 Mouse
    • SwissProt: Q9XSW2 Pig
    • SwissProt: Q62986 Rat
    • SwissProt: Q9TU15 Sheep
    see all
  • Alternative names

    • ER-BETA antibody
    • Erb antibody
    • ESR-BETA antibody
    • ESRB antibody
    • ESTRB antibody
    • Estrogen nuclear receptor beta variant a antibody
    • Estrogen nuclear receptor beta variant b antibody
    • Estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.

  • Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
    Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
    Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Predicted band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Gastric Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Bladder Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.

  • Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
    Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

    Flow cytometry analysis of  BT474 (human ductal breast epithelial tumor cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Grey-isotype control,Green-AF4888-labeled ER beta 1.

  • Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
    Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)

    Flow cytometry analysis of MCF-7 (human breast adenocarcinoma cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Green-isotype control, Red-PE labeled ER-beta 1.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab187291? Please let us know so that we can cite the reference in this datasheet.

ab187291 has been referenced in 3 publications.

  • Padma AM  et al. Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning. NPJ Regen Med 6:26 (2021). PubMed: 34021161
  • Tiemann TT  et al. Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation. Mol Hum Reprod 26:167-178 (2020). PubMed: 31980817
  • Zhang L  et al. Estrogen Receptor ß1 Expression Patterns Have Different Effects on Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors' Treatment Response in Epidermal Growth Factor Receptor Mutant Lung Adenocarcinoma. Front Oncol 10:603883 (2020). PubMed: 33585221

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (fetal sheep cells)
Permeabilization
No
Specification
fetal sheep cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 29 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (sheep uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citric acid,pH 6.0, 0,05% Tween 20
Permeabilization
Yes - Triton-X100 0.2%
Specification
sheep uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 29 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 05 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 31 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (vagina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
vagina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 21 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (oviduct)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
oviduct
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 15 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 15 2015

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