Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-220890-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)")
Key features and details
- Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Sheep, Horse, Human, Pig, Monkey
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal -
Description
Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal -
Host species
Mouse -
Specificity
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics. -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Horse, Human, Pig, Monkey -
Immunogen
Recombinant fragment corresponding to Human Estrogen Receptor beta 1 (C terminal).
Database link: Q92731 -
Positive control
- Flow Cyt: BT474 and MCF-7 cells IHC-P: Ovarian carcinoma, breast carcinoma tissues, gastric carcinoma, and bladder carcinoma tissues. WB: MCF7 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab187291 was purified from Bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
ERb455 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa.
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| IHC-P | (6) |
Use a concentration of 1 - 2 µg/ml.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
| Flow Cyt |
Use 1-2µg for 106 cells.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
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Flow Cyt
Use 1-2µg for 106 cells. |
Target
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Relevance
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 100033964 Horse
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Entrez Gene: 396697 Pig
- Entrez Gene: 25149 Rat
- Entrez Gene: 443066 Sheep
- Omim: 601663 Human
- SwissProt: Q92731 Human
see all -
Alternative names
- ER-BETA antibody
- Erb antibody
- ESR-BETA antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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![Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-454709-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-western-blot-mcf7-human.jpg)
Western blot - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 59 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-220889-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-252565-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Gastric Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-252568-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Bladder Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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![Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-251289-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Flow cytometry analysis of BT474 (human ductal breast epithelial tumor cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Grey-isotype control,Green-AF4888-labeled ER beta 1.
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![Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)](https://www.abcam.com/ps/products/187/ab187291/Images/ab187291-251287-anti-estrogen-receptor-beta-1-antibody-erb455-c-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291)Flow cytometry analysis of MCF-7 (human breast adenocarcinoma cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Green-isotype control, Red-PE labeled ER-beta 1.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab187291 has been referenced in 4 publications.
- Xu Z et al. Positive interaction between GPER and β-alanine in the dorsal root ganglion uncovers potential mechanisms: mediating continuous neuronal sensitization and neuroinflammation responses in neuropathic pain. J Neuroinflammation 19:164 (2022). PubMed: 35729568
- Padma AM et al. Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning. NPJ Regen Med 6:26 (2021). PubMed: 34021161
- Tiemann TT et al. Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation. Mol Hum Reprod 26:167-178 (2020). PubMed: 31980817
- Zhang L et al. Estrogen Receptor ß1 Expression Patterns Have Different Effects on Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors' Treatment Response in Epidermal Growth Factor Receptor Mutant Lung Adenocarcinoma. Front Oncol 10:603883 (2020). PubMed: 33585221