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Anti-Estrogen Receptor beta antibody (ab5786)

Reviews (1)Q&A (2)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody (ab5786)

    Key features and details

    • Rabbit polyclonal to Estrogen Receptor beta
    • Suitable for: IHC-P
    • Reacts with: Rat
    • Isotype: IgG

    Overview

    • Product name

      Anti-Estrogen Receptor beta antibody
      See all Estrogen Receptor beta primary antibodies

    • Description

      Rabbit polyclonal to Estrogen Receptor beta

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Rat

    • Immunogen

      Synthetic peptide corresponding to Human Estrogen Receptor beta aa 459-477.
      Sequence:

      CSPAEDSKSKEGSQNPQSQ


      (Peptide available as ab41764)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab5786 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

    • Tissue specificity

      Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.

    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • Domain

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • ER BETA antibody
        • ER-beta antibody
        • Erb antibody
        • ESR B antibody
        • ESR BETA antibody
        • ESR2 antibody
        • ESR2_HUMAN antibody
        • ESRB antibody
        • ESTRB antibody
        • estrogen nuclear receptor beta variant a antibody
        • estrogen nuclear receptor beta variant b antibody
        • estrogen receptor 2 (ER beta) antibody
        • Estrogen receptor 2 antibody
        • estrogen receptor beta 4 antibody
        • Estrogen receptor beta antibody
        • NR3A2 antibody
        • Nuclear receptor subfamily 3 group A member 2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody (ab5786)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody (ab5786)

        Immunohistochemical staining with ab5786 detects nuclear Estrogen Receptor beta in rat prostate

      References (5)

      Publishing research using ab5786? Please let us know so that we can cite the reference in this datasheet.

      ab5786 has been referenced in 5 publications.

      • Kamal DAM  et al. Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model. Int J Mol Sci 23:N/A (2022). PubMed: 36499085
      • Li Y  et al. Cell sex affects extracellular matrix protein expression and proliferation of smooth muscle progenitor cells derived from human pluripotent stem cells. Stem Cell Res Ther 8:156 (2017). PubMed: 28676082
      • Peng J  et al. Expression of voltage-gated sodium channel Nav1.5 in non-metastatic colon cancer and its associations with estrogen receptor (ER)-ß expression and clinical outcomes. Chin J Cancer 36:89 (2017). IHC-P ; Human . PubMed: 29122010
      • Lloyd MC  et al. Vascular measurements correlate with estrogen receptor status. BMC Cancer 14:279 (2014). IHC-P ; Human . PubMed: 24755315
      • Anagnostis A & Papadopoulos AI Effects of a diet rich in sesame ( Sesamum indicum) pericarp on the expression of oestrogen receptor alpha and oestrogen receptor beta in rat prostate and uterus. Br J Nutr 102:703-8 (2009). WB ; Rat . PubMed: 19309532

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor beta antibody

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Human breast cancer xenograft(MCF-7))
      Specification
      Human breast cancer xenograft(MCF-7)
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH7.0
      Permeabilization
      No
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Sep 17 2010

      Question

      ... Please, how many glass slides can be stained with 1 bottle of 1 ml?
      I have 200 glass slides with lung tissue.

      Thank you.
      Sincerely,

      Read More
      Answer

      Thank you for your inquiry. My colleague will send you a proforma invoice shortly.

      In regards to your question of staining: One vial of ab5786 contains 50ul. The optimal dilution should be determined in your laboratory. Lets suppose you will have a nice staining with a dilution of 1/50, you would have 2.5ml of staining solution. If the antibody does work well with a dilution of 1/100 in your hands, you will have 5ml of staining solution.

      Depending on the size of your slide, you will need to use either 50ul or 100ul of staining solution per slide. If it is a big tissue on the slide, it might even could be 200ul. I recommend to check what volume is optimal to cover well the tissue without risk of drying.
      If you would need 100ul of staining solution per slide, you would need 20ml of staining solution for 200 slides.

      I am sorry that I can not give you a definite answer. It depends on the final dilution factor in your hands as well as on the size of the tissue.

      I hope this information is nevertheless helpful. Please do not hesitate to contact us again, should you have any additional question.

      Read More

      Question

      Our customer had tried to stain their rat samples without any luck. They have used paraffin embedded formalin-fixed rat prostate samples. They have done heat retrieval (microwave oven, 15 min. citrate puffer, pH6) Dilutions for #ab5786 were 1:50, 1:100 and 1:200, o/n +4C. The results are no staining at all. I hope you could give me some good advice about the matter. Thank you in advance for your help.

      Read More
      Answer

      Thank you for your enquiry. I'm sorry ab5786 is giving your customer difficulty. From the protocol you give me I think the antigen retrieval method is fine and the incubation time/temperature for the primary antibody is fine too. I would check whether the buffers used to dilute the antibodies contain triton 0.3%v/v, as this greatly helps the penetration of the antibody in the sections. Does your customer have a positive control? Does his secondary antibody work with other antibodies in the lab, or could it have a problem? Please let me know if you need further advice, I hope this helps,

      Read More

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