Anti-ETFDH antibody [3H2BG1] (ab126576)
![Western blot - Anti-ETFDH antibody [3H2BG1] (ab126576)](https://www.abcam.com/ps/products/126/ab126576/Images/ab126576-140519-anti-etfdh-antibody-3h2bg1-western-blot.jpg "Western blot - Anti-ETFDH antibody [3H2BG1] (ab126576)")
Key features and details
- Mouse monoclonal [3H2BG1] to ETFDH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-ETFDH antibody [3H2BG1]
See all ETFDH primary antibodies -
Description
Mouse monoclonal [3H2BG1] to ETFDH -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Human cell, heart and liver lysates, Rat liver lysates, Mouse liver lysates, Bovine heart mitochondria lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. Near homogeneity as judged by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
3H2BG1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. |
|
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Accepts electrons from ETF and reduces ubiquinone. -
Involvement in disease
Defects in ETFDH are the cause of glutaric aciduria type 2C (GA2C) [MIM:231680]. GA2C is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. -
Sequence similarities
Belongs to the ETF-QO/fixC family.
Contains 1 4Fe-4S ferredoxin-type domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 768074 Cow
- Entrez Gene: 2110 Human
- Entrez Gene: 66841 Mouse
- Entrez Gene: 295143 Rat
- Omim: 231675 Human
- SwissProt: Q2KIG0 Cow
- SwissProt: Q16134 Human
- SwissProt: Q921G7 Mouse
see all -
Alternative names
- Electron transfer flavoprotein ubiquinone oxidoreductase antibody
- Electron transfer flavoprotein-ubiquinone oxidoreductase antibody
- electron transferring flavoprotein dehydrogenase antibody
see all
Images
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Western blot - Anti-ETFDH antibody [3H2BG1] (ab126576)All lanes : Anti-ETFDH antibody [3H2BG1] (ab126576) at 1 µg/ml
Lane 1 : Human heart lysate at 20 µg
Lane 2 : Human liver lysate at 20 µg
Lane 3 : Human cell lysate at 20 µg
Lane 4 : Rat liver lysate at 20 µg
Lane 5 : Mouse liver lysate at 20 µg
Lane 6 : Bovine heart mitochondria
at 10 µg
Secondary
All lanes : GAM-HRP at 1/3000 dilution
Predicted band size: 68 kDa
The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the goat anti-Mouse secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody [3H2BG1] (ab126576)](https://www.abcam.com/ps/products/126/ab126576/Images/ab126576-140518-anti-etfdh-antibody-3h2bg1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody [3H2BG1] (ab126576)IHC image of ETFDH staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126576, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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Datasheet download
References (4)
ab126576 has been referenced in 4 publications.
- Fisher-Wellman KH et al. Respiratory Phenomics across Multiple Models of Protein Hyperacylation in Cardiac Mitochondria Reveals a Marginal Impact on Bioenergetics. Cell Rep 26:1557-1572.e8 (2019). PubMed: 30726738
- Zhu M et al. Riboflavin-responsive multiple Acyl-CoA dehydrogenation deficiency in 13 cases, and a literature review in mainland Chinese patients. J Hum Genet N/A:N/A (2014). Human . PubMed: 24522293
- Cornelius N et al. Secondary coenzyme Q10 deficiency and oxidative stress in cultured fibroblasts from patients with riboflavin responsive multiple Acyl-CoA dehydrogenation deficiency. Hum Mol Genet 22:3819-27 (2013). WB ; Human . PubMed: 23727839
- Orr AL et al. A Refined Analysis of Superoxide Production by Mitochondrial sn-Glycerol 3-Phosphate Dehydrogenase. J Biol Chem 287:42921-35 (2012). WB . PubMed: 23124204
