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    products/primary-antibodies/ezrin-antibody-ep886y-plasma-membrane-marker-ab40839.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

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  • SDS
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Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Flow Cytometry (Intracellular) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunoprecipitation - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Flow Cytometry (Intracellular) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
  • Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP886Y] to Ezrin - Plasma Membrane Marker
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free HRP

You may also be interested in

Protein
Product image
Recombinant Human Ezrin protein (ab132942)
Primary
Product image
Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (ab198520)

View more associated products

Overview

  • Product name

    Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker
    See all Ezrin primary antibodies
  • Description

    Rabbit monoclonal [EP886Y] to Ezrin - Plasma Membrane Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra), WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Ezrin aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate; HeLa and HCT116 whole cell lysates. IHC-P: Human Colon, breast and bladder carcinoma. ICC/IF: HeLa cells Flow Cyt (intra): HeLa cells IP: HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP886Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (ab198520)
    • HRP Anti-Ezrin antibody [EP886Y] (ab198522)
    • Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human Ezrin protein (ab132942)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
1/500.

For upurified use at 1/50 - 1/100

Flow Cyt (Intra)
1/800.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For upurified use at 1/1000

WB (2)
1/5000 - 1/50000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).
IP
1/40.

For upurified use at 1/50

IHC-P (1)
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
ICC/IF
1/500.

For upurified use at 1/50 - 1/100

Flow Cyt (Intra)
1/800.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For upurified use at 1/1000

WB
1/5000 - 1/50000. Detects a band of approximately 72 kDa (predicted molecular weight: 69 kDa).
IP
1/40.

For upurified use at 1/50

IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • Tissue specificity

    Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • Sequence similarities

    Contains 1 FERM domain.
  • Developmental stage

    Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • Post-translational
    modifications

    Phosphorylated by tyrosine-protein kinases.
  • Cellular localization

    Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Target information above from: UniProt accession P15311 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7430 Human
    • Omim: 123900 Human
    • SwissProt: P15311 Human
    • Unigene: 487027 Human
    • Alternative names

      • Villin 2 ezrin antibody
      • CVIL antibody
      • CVL antibody
      • Cytovillin 2 antibody
      • Cytovillin antibody
      • DKFZp762H157 antibody
      • Epididymis secretory protein Li 105 antibody
      • EZR antibody
      • EZRI_HUMAN antibody
      • Ezrin antibody
      • FLJ26216 antibody
      • HEL S 105 antibody
      • MGC1584 antibody
      • p81 antibody
      • VIL 2 antibody
      • VIL2 antibody
      • Villin 2 (ezrin) antibody
      • Villin 2 antibody
      • Villin-2 antibody
      • Villin2 antibody
      see all

    Images

    • Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Ezrin knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HCT116 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab40839 observed at 75 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab40839 was shown to specifically react with Ezrin in wild-type HAP1 cells as signal was lost in Ezrin knockout cells. Wild-type and Ezrin knockout samples were subjected to SDS-PAGE. Ab40839 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Ezrin with Purified ab40839 at 1:250 dilution (3.28 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ezrin with Purified ab40839 at 1:500 dilution (1.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      All lanes : Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839) at 1/20000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 69 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling Ezrin with Purified ab40839 at 1:250 dilution (3.28 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Flow Cytometry (Intracellular) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Flow Cytometry (Intracellular) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ezrin with Purified ab40839 at 1/800 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunoprecipitation - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunoprecipitation - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      ab40839 (purified) at 1:40 dilution (2µg) immunoprecipitating Ezrin in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab40839 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40839 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Flow Cytometry (Intracellular) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Overlay histogram showing SH-SY5Y cells stained with upurifiedab40839 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40839, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      ICC/IF image of unpurified ab40839 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab40839 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839) at 1/50000 dilution (Unpurified) + Hela cell lysate at 10 µg

      Predicted band size: 69 kDa
      Observed band size: 72 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Unpurified ab40839 at a 1:100 dilution staining Ezrin in human colon carcinoma tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Fluorescent immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using unpurified ab40839. Green-Ezrin red-PI

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Unpurified ab40839 showing positive staining in Breast carcinoma tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Unpurified ab40839 showing positive staining in Prostatic carcinoma tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Unpurified ab40839 showing positive staining in Hepatocellular carcinoma tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

      Unpurified ab40839 showing positive staining in Normal kidney tissue.

    • Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)
      Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (ab40839)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab40839? Please let us know so that we can cite the reference in this datasheet.

    ab40839 has been referenced in 10 publications.

    • Kim J  et al. Development of 3D Printed Bruch's Membrane-Mimetic Substance for the Maturation of Retinal Pigment Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33499245
    • Zhang C  et al. Targeting POLE2 Creates a Novel Vulnerability in Renal Cell Carcinoma via Modulating Stanniocalcin 1. Front Cell Dev Biol 9:622344 (2021). PubMed: 33644060
    • Zhang L  et al. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22:851 (2021). PubMed: 34149897
    • Zhao J  et al. Drug resistance gene expression and chemotherapy sensitivity detection in Chinese women with different molecular subtypes of breast cancer. Cancer Biol Med 17:1014-1025 (2020). PubMed: 33299650
    • Schumacher D  et al. Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3D cultures. PLoS Genet 15:e1008076 (2019). PubMed: 30925167
    • Zhang Y & Wang G MicroRNA-183 inhibits A375 human melanoma cell migration and invasion by targeting Ezrin and MMP-9. Oncol Lett 17:548-554 (2019). PubMed: 30655800
    • Yan H  et al. Upregulation of miR-183-5p is responsible for the promotion of apoptosis and inhibition of the epithelial-mesenchymal transition, proliferation, invasion and migration of human endometrial cancer cells by downregulating Ezrin. Int J Mol Med 42:2469-2480 (2018). PubMed: 30226564
    • Ma L & Jiang T Clinical implications of Ezrin and CD44 co-expression in breast cancer. Oncol Rep 30:1899-905 (2013). PubMed: 23900701
    • Grzendowski M  et al. Differential proteome analysis of human gliomas stratified for loss of heterozygosity on chromosomal arms 1p and 19q. Neuro Oncol 12:243-56 (2010). PubMed: 20167812
    • Wang G  et al. MicroRNA-183 regulates Ezrin expression in lung cancer cells. FEBS Lett 582:3663-8 (2008). PubMed: 18840437

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2Os)
    Permeabilization
    Yes - Tx100
    Specification
    U2Os
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Jerome Cattin

    Verified customer

    Submitted Jun 25 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - 0.5% triton-X100
    Specification
    U2OS
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Lawrence Welch

    Verified customer

    Submitted Jun 17 2020

    Western blot abreview for Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Jerome Cattin

    Verified customer

    Submitted May 20 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ezrin antibody [EP886Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain cortex)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C 20 min
    Permeabilization
    No
    Specification
    brain cortex
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 16 2015

    Western blot abreview for Anti-Ezrin antibody [EP886Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human brain)
    Loading amount
    10 µg
    Specification
    Human brain
    Treatment
    Lysis buffer (urea/thiourea)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 03 2010

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