Anti-Ezrin antibody [EP924Y] (ab75840)
![Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)](https://www.abcam.com/ps/products/75/ab75840/Images/ab75840-448926-anti-ezrin-antibody-ep924y-western-blot-hela-lysates.jpg "Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)")
Key features and details
- Rabbit monoclonal [EP924Y] to Ezrin
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-Ezrin antibody [EP924Y]
See all Ezrin primary antibodies -
Description
Rabbit monoclonal [EP924Y] to Ezrin -
Host species
Rabbit -
Specificity
ab75840 detects both phosphorylated and non phosphorylated Ezrin.
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Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HeLa, Wild-type HAP1, HeLa, HCT116, 293, A431 and SH-SY5Y cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP924Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
Tissue specificity
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
Sequence similarities
Contains 1 FERM domain. -
Developmental stage
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinases. -
Cellular localization
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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Database links
- Entrez Gene: 7430 Human
- Entrez Gene: 22350 Mouse
- Entrez Gene: 54319 Rat
- Omim: 123900 Human
- SwissProt: P15311 Human
- SwissProt: P26040 Mouse
- SwissProt: P31977 Rat
- Unigene: 487027 Human
see all -
Alternative names
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
Images
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Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)All lanes : Anti-Ezrin antibody [EP924Y] (ab75840) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : EZR knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab75840 observed at 75 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab75840 was shown to react with Ezrin in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264912 (knockout cell lysate ab256908) was used. Wild-type HeLa and EZR knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab75840 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1/1000 dilution and a 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)](https://www.abcam.com/ps/products/75/ab75840/Images/ab75840-296573-anti-ezrin-antibody-ep924y-western-blot.jpg)
Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)All lanes : Anti-Ezrin antibody [EP924Y] (ab75840) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Ezrin knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HCT116 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 69 kDaLanes 1 - 4: Merged signal (red and green). Green - ab75840 observed at 75 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab75840 was shown to specifically react with Ezrin in wild-type HAP1 cells as signal was lost in Ezrin knockout cells. Wild-type and Ezrin knockout samples were subjected to SDS-PAGE. Ab75840 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)](https://www.abcam.com/ps/products/75/ab75840/Images/ab75840-90963-anti-ezrin-antibody-ep924y-western-blot.gif)
Western blot - Anti-Ezrin antibody [EP924Y] (ab75840)All lanes : Anti-Ezrin antibody [EP924Y] (ab75840) at 1/10000 dilution
Lane 1 : 293 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 69 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab75840 has been referenced in 5 publications.
- Liu H et al. Increased expression of IFN-? in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop. Exp Ther Med 21:112 (2021). PubMed: 33335575
- Li Q et al. Role of mTOR signaling in the regulation of high glucose-induced podocyte injury. Exp Ther Med 17:2495-2502 (2019). PubMed: 30906437
- Röwer C et al. Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues. Transl Oncol 11:1-10 (2017). WB ; Human . PubMed: 29132012
- Watanabe K et al. Participation of ezrin in bacterial uptake by trophoblast giant cells. Reprod Biol Endocrinol 7:95 (2009). PubMed: 19737422
- Wang G et al. MicroRNA-183 regulates Ezrin expression in lung cancer cells. FEBS Lett 582:3663-8 (2008). PubMed: 18840437