Anti-Ezrin (phospho T567) antibody (ab47293)
Key features and details
- Rabbit polyclonal to Ezrin (phospho T567)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ezrin (phospho T567) antibody
See all Ezrin primary antibodies -
Description
Rabbit polyclonal to Ezrin (phospho T567) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Ezrin only when phosphorylated at Threonine 567. This antibody will also react with Moesin at Threonine 577 and Radixin at Threonine 564. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Ezrin (phospho T567).
Database link: P15311 -
Positive control
- Extracts from A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was purified using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. |
Target
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Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
Tissue specificity
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
Sequence similarities
Contains 1 FERM domain. -
Developmental stage
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
Post-translational
modificationsPhosphorylated by tyrosine-protein kinases. -
Cellular localization
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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Database links
- Entrez Gene: 7430 Human
- Entrez Gene: 22350 Mouse
- Entrez Gene: 54319 Rat
- Omim: 123900 Human
- SwissProt: P15311 Human
- SwissProt: P26040 Mouse
- SwissProt: P31977 Rat
- Unigene: 487027 Human
see all -
Alternative names
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
Images
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Western blot - Anti-Ezrin (phospho T567) antibody (ab47293)All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)
Lane 1 : EGF treated A431 cells, no added peptide
Lane 2 : Untreated A431 cells, no added peptide
Predicted band size: 69 kDa
Observed band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (phospho T567) antibody (ab47293)Ab47293 staining human normal placenta. Staining is localised to apical membrane and cytoplasmic compartiment.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may b -
Western blot - Anti-Ezrin (phospho T567) antibody (ab47293)All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)
Lane 1 : Jurkat cell lysate with PMA (125 ng/ml, 30 minutes)
Lane 2 : Jurkat cell lysate
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 68 kDa (possible cross reactivity)
The lower band is likely to be Moesin, which has a homologous immunogenic region.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab47293 has been referenced in 16 publications.
- Li F et al. Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation. Int J Biol Sci 18:1238-1253 (2022). PubMed: 35173550
- Tanaka C et al. Ezrin Modulates the Cell Surface Expression of Programmed Cell Death Ligand-1 in Human Cervical Adenocarcinoma Cells. Molecules 26:N/A (2021). PubMed: 34577118
- Broadhead MJ et al. Nanostructural Diversity of Synapses in the Mammalian Spinal Cord. Sci Rep 10:8189 (2020). PubMed: 32424125
- Chetry M et al. Effects of Galectin-1 on Biological Behavior in Cervical Cancer. J Cancer 11:1584-1595 (2020). PubMed: 32047564
- Leiphrakpam PD et al. TGFß and IGF1R signaling activates protein kinase A through differential regulation of ezrin phosphorylation in colon cancer cells. J Biol Chem 293:8242-8254 (2018). PubMed: 29599290