Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

Reviews (2) Submit a question References (10)
Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Dot Blot - Anti-Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) antibody [EP2122Y] (ab76247)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]
    See all Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) primary antibodies

  • Description

    Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558)

  • Host species

    Rabbit

  • Specificity

    ab76247 will detect Ezrin, Radixin and Moesin. This antibody detects the phosphorylated target and not the non-phosphorylated epitope.

  • Tested applications

    Suitable for: WB, IHC-P, Dot blotmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558). The exact sequence is proprietary. A synthetic peptide corresponding to residues surrounding Thr567 (Ezrin), Thr564 (Radixin) and Thr558 (Moesin) of human Ezrin.

  • Positive control

    • WB: HeLa, HEK-293, NIH/3T3, and C6 starved overnight, then treated with 100nM Calyculin A whole cell lysates. IHC-P: Human breast, mouse testis, and rat liver tissues. Dot Blot: Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) phospho peptide and Non-phospho peptide.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 75-81 kDa (predicted molecular weight: 69 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot
1/1000.
Notes
WB
1/1000. Detects a band of approximately 75-81 kDa (predicted molecular weight: 69 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot
1/1000.
Application notes
Is unsuitable for Flow Cyt.

Images

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
    Lane 4 : Untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) starved overnight, then treated with 100nM Calyculin A for 60 minutes whole cell lysate
    Lane 6 : HEK-293 (Human embryonic kidney epithelial cell) starved overnight, then treated with 100nM Calyculin A for g0 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyonic fibroblast) whole cell lysate
    Lane 2 : Untreated NIH/3T3 (Mouse embyonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : Untreated NIH/3T3 (Mouse embyonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 2 : Untreated C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes whole cell lysate
    Lane 3 : Untreated C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Dot Blot - Anti-Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) antibody [EP2122Y] (ab76247)
    Dot Blot - Anti-Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) antibody [EP2122Y] (ab76247)

    Dot blot analysis of Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) phospho peptide (Lane 1), Non-phospho peptide (Lane 2), labelling Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) with ab76247 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The sequence of non-phospho peptide: CGRDKYKTLRQIR.
    The sequence of phospho peptide: CGRDKYK-pT-LRQIR.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (10)

Publishing research using ab76247? Please let us know so that we can cite the reference in this datasheet.

ab76247 has been referenced in 10 publications.

  • Wang X  et al. Formative pluripotent stem cells show features of epiblast cells poised for gastrulation. Cell Res 31:526-541 (2021). PubMed: 33608671
  • Zhang L  et al. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22:851 (2021). PubMed: 34149897
  • Li Y  et al. CircGLIS3 Promotes High-Grade Glioma Invasion via Modulating Ezrin Phosphorylation. Front Cell Dev Biol 9:663207 (2021). PubMed: 34540823
  • Zhang L  et al. Knockout of STK10 promotes the migration and invasion of cervical cancer cells. Transl Cancer Res 9:7079-7090 (2020). PubMed: 35117313
  • Guo C  et al. Bilobalide reversibly modulates blood-brain barrier permeability through promoting adenosine A1 receptor-mediated phosphorylation of actin-binding proteins. Biochem Biophys Res Commun 526:1077-1084 (2020). PubMed: 32312522
  • Mu L  et al. A phosphatidylinositol 4,5-bisphosphate redistribution-based sensing mechanism initiates a phagocytosis programing. Nat Commun 9:4259 (2018). PubMed: 30323235
  • Lee A  et al. Vitamin D Proliferates Vaginal Epithelium through RhoA Expression in Postmenopausal Atrophic Vagina tissue. Mol Cells 40:677-684 (2017). WB ; Human . PubMed: 28843271
  • Luján P  et al. PRL-3 disrupts epithelial architecture by altering the post-mitotic midbody position. J Cell Sci 129:4130-4142 (2016). PubMed: 27656108
  • Geisler F  et al. A novel function for the MAP kinase SMA-5 in intestinal tube stability. Mol Biol Cell 27:3855-3868 (2016). WB ; Caenorhabditis elegans . PubMed: 27733627
  • Lööv C  et al. Identification of injury specific proteins in a cell culture model of traumatic brain injury. PLoS One 8:e55983 (2013). WB, ICC/IF ; Mouse . PubMed: 23409102

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

Filter by Application

Filter by Species

Filter by Ratings

1-2 of 2 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Permeabilization
Yes - 0.3% Triton
Specification
HeLa Cells
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 19 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.3% Triton X-100
Specification
HeLa
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 09 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com