Anti-Factor H antibody [OX-24] (ab118820)
![Western blot - Anti-Factor H antibody [OX-24] (ab118820)](https://www.abcam.com/ps/products/118/ab118820/Images/ab118820-352433-ab118820AP46633483pcMilkPublish.jpg "Western blot - Anti-Factor H antibody [OX-24] (ab118820)")
Key features and details
- Mouse monoclonal [OX-24] to Factor H
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Factor H antibody [OX-24]
See all Factor H primary antibodies -
Description
Mouse monoclonal [OX-24] to Factor H -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Factor H.
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Positive control
- WB: Human plasma, Human serum, Purified Factor H protein
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General notes
ab118820 has switched from ascites to TCS on 19th September 2019. Lot numbers higher than GR3258447 are from tissue culture supernatant.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OX-24 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 10 µg/ml.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.
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| Notes |
|---|
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ELISA
Use a concentration of 10 µg/ml. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa. |
Target
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Function
Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Genetic variations in CFH are associated with basal laminar drusen (BLD) [MIM:126700]; also known as drusen of Bruch membrane or cuticular drusen or grouped early adult-onset drusen. Drusen are extracellular deposits that accumulate below the retinal pigment epithelium on Bruch membrane. Basal laminar drusen refers to an early adult-onset drusen phenotype that shows a pattern of uniform small, slightly raised yellow subretinal nodules randomly scattered in the macula. In later stages, these drusen often become more numerous, with clustered groups of drusen scattered throughout the retina. In time these small basal laminar drusen may expand and ultimately lead to a serous pigment epithelial detachment of the macula that may result in vision loss.
Defects in CFH are the cause of complement factor H deficiency (CFH deficiency) [MIM:609814]. CFH deficiency determines uncontrolled activation of the alternative complement pathway with consumption of C3 and often other terminal complement components. It is associated with a number of renal diseases with variable clinical presentation and progression, including membranoproliferative glomerulonephritis and atypical hemolytic uremic syndrome. CFH deficiency patients may show increased susceptibility to meningococcal infections.
Defects in CFH are a cause of susceptibility to hemolytic uremic syndrome atypical type 1 (AHUS1) [MIM:235400]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Genetic variation in CFH is associated with age-related macular degeneration type 4 (ARMD4) [MIM:610698]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
Sequence similarities
Contains 20 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3075 Human
- Omim: 134370 Human
- SwissProt: P08603 Human
- Unigene: 363396 Human
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Alternative names
- adrenomedullin binding protein antibody
- age related maculopathy susceptibility 1 antibody
- AHUS 1 antibody
see all
Images
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Western blot - Anti-Factor H antibody [OX-24] (ab118820)All lanes : Anti-Factor H antibody [OX-24] (ab118820) at 1 µg/ml
Lane 1 : Human serum diluted 1/100 at 10 µl
Lane 2 : Human plasma diluted 1/100 at 10 µl
Lane 3 : Purified Factor H protein at 0.5 µg
Lane 4 : Purified Factor H protein at 0.1 µg
Performed under reducing conditions.
Predicted band size: 139 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab118820 overnight at 4°C at a 1ug/ml concentration. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution for 1 hour at room temperature before imaging.
This image was generated using the ascites version of the product.
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![ELISA - Anti-Factor H antibody [OX-24] (ab118820)](https://www.abcam.com/ps/products/118/ab118820/Images/ab118820-4-ab118820-AP4663348-ELISA007.jpg)
ELISA - Anti-Factor H antibody [OX-24] (ab118820)96-well microtitre plates were coated overnight at 4°C with recombinant human C3, C3b, iC3b, and Factor H proteins, in duplicate at a concentration of 1µg/mL. Plates were blocked with 1% BSA in PBS-T (0.1% Tween®) for 1 hour before incubation with a 10-step 4x serial dilution of ab118820 from 10µg/mL for 1 hour at room temperature. Antibody binding was detected with Goat Anti-Mouse IgG H&L (HRP) (ab6789) secondary antibody at a 1 in 10000 dilution for 1 hour at room temperature. Plates were incubated with TMB ELISA substrate for 7 minutes prior to being stopped with Stop solution and absorbance measured at 450nm.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab118820 has been referenced in 6 publications.
- Sun Y et al. Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria. Front Cell Infect Microbiol 10:592906 (2020). PubMed: 33569353
- Chen X et al. Cadmium Induces Glomerular Endothelial Cell-Specific Expression of Complement Factor H via the -1635 AP-1 Binding Site. J Immunol 202:1210-1218 (2019). PubMed: 30642982
- Medjeral-Thomas NR et al. Glomerular Complement Factor H-Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment. Kidney Int Rep 4:1387-1400 (2019). PubMed: 31701048
- Liu J & Hoh J Loss of Complement Factor H in Plasma Increases Endothelial Cell Migration. J Cancer 8:2184-2190 (2017). WB ; Mouse . PubMed: 28819420
- Popek S et al. IL-6 and IL-8 enhance factor H binding to the cell membranes. Mol Med Rep 13:3886-94 (2016). IF . PubMed: 27035765
- Lee MS et al. Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection. PLoS Pathog 10:e1004412 (2014). ICC/IF ; Human . PubMed: 25254972