Recombinant Anti-Factor X antibody [EPR16249] (ab196023)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16249] to Factor X
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Factor X antibody [EPR16249]
See all Factor X primary antibodies -
Description
Rabbit monoclonal [EPR16249] to Factor X -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, Human blood, Human plasma and HepG2 lysates; Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16249 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/20000. Detects a band of approximately 74 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
1/1200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/20000. Detects a band of approximately 74 kDa (predicted molecular weight: 54 kDa). |
IHC-P
1/1200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
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Form
Cleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain -
Alternative names
- Activated factor Xa heavy chain antibody
- Coagulation factor X antibody
- F10 antibody
see all
Images
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All lanes : Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human blood lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
We hypothesis the 74 kDa band is the precursor of Factor X.
Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479. -
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Factor X with ab196023 at 1/1200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution + Human plasma lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
We hypothesis the 74 kDa band is the precursor of Factor X.
Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479. -
Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
We hypothesis the 74 kDa band is the precursor of Factor X.
Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab196023 has not yet been referenced specifically in any publications.