Recombinant Anti-FAK antibody [EP695Y] (ab40794)
![Western blot - Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-11-anti-fak-antibody-ep695y-western-blot-human-lysate.jpg "Western blot - Anti-FAK antibody [EP695Y] (ab40794)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP695Y] to FAK
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FAK antibody [EP695Y]
See all FAK primary antibodies -
Description
Rabbit monoclonal [EP695Y] to FAK -
Host species
Rabbit -
Specificity
ab40794 recognises Focal adhesion kinase (FAK).
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3, HeLa, A431, HEK-293T and K-562 cell lysates; Rat tissue lysate. IHC-P: Human hepatocellular carcinoma.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP695Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow. |
| WB | (3) |
1/2000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa).
For unpurified use at 1/1000 |
| Notes |
|---|
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IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow. |
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WB
1/2000. Detects a band of approximately 125 kDa (predicted molecular weight: 119 kDa). For unpurified use at 1/1000 |
Target
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Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
Tissue specificity
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
Domain
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
Post-translational
modificationsPhosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
Cellular localization
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
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Database links
- Entrez Gene: 506270 Cow
- Entrez Gene: 5747 Human
- Entrez Gene: 14083 Mouse
- Entrez Gene: 25614 Rat
- Omim: 600758 Human
- SwissProt: Q05397 Human
- SwissProt: P34152 Mouse
- SwissProt: O35346 Rat
see all -
Alternative names
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
Images
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Western blot - Anti-FAK antibody [EP695Y] (ab40794)All lanes : Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : PTK2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 119 kDaLanes 1 - 4: Merged signal (red and green). Green - ab40794 observed at 119 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab40794 was shown to react with FAK in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255421 (knockout cell lysate ab263766) was used. Wild-type and FAK knockout samples were subjected to SDS-PAGE. ab40794 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-337469-anti-fak-antibody-ep695y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1:250 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Western blot - Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-337518-anti-fak-antibody-ep695y-western-blot.jpg)
Western blot - Anti-FAK antibody [EP695Y] (ab40794)Lane 1 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution (Purified)
Lane 2 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 119 kDa -
![Western blot - Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-337517-anti-fak-antibody-ep695y-western-blot.jpg)
Western blot - Anti-FAK antibody [EP695Y] (ab40794)Lane 1 : Anti-FAK antibody [EP695Y] (ab40794) at 1/2000 dilution (Purified)
Lane 2 : Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 119 kDa
Observed band size: 119 kDa -
![Western blot - Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-53983-anti-fak-antibody-ep695y-western-blot.jpg)
Western blot - Anti-FAK antibody [EP695Y] (ab40794)Anti-FAK antibody [EP695Y] (ab40794) at 1/1000 dilution + Hela cell lysate
Predicted band size: 119 kDa
Observed band size: 119 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-53982-anti-fak-antibody-ep695y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-53980-anti-fak-antibody-ep695y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (ab40794)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomThe image shows FAK antibody (ab40794) in human spleen tissue. Clear cytoplasmic positivity in a subset of germinal centre cells. The there is intense positivity of the serum in the blood vessels. Endogenous peroxidases was blocked using 2% H2O2, for 15 minutes. -
![Western blot - Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-276775-anti-fak-antibody-ep695y-western-blot.jpg)
Western blot - Anti-FAK antibody [EP695Y] (ab40794)This image is courtesy of an Abreview submitted by Magali Boissiere (6970246)All lanes : Anti-FAK antibody [EP695Y] (ab40794)
All lanes : Milk PBS Tween
Blocking peptides at 5 % per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit poly clonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesWestern blot analysis of RAW264.7 cells lysate (40μg/lane) labelling FAK with ab40794 at 1/5000 in 5% Milk PBS Tween for 16 hours at 4ºC. A HRP conjugated goat anti-rabbit poly clonal (1/5000) was used as the secondary antibody.
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![Anti-FAK antibody [EP695Y] (ab40794)](https://www.abcam.com/ps/products/40/ab40794/Images/ab40794-13-benefits-of-recombinant-antibodies.png)
Anti-FAK antibody [EP695Y] (ab40794)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (132)
ab40794 has been referenced in 132 publications.
- Yu L et al. Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway. Bioact Mater 20:598-609 (2023). PubMed: 35846837
- Hu X et al. Overexpression of DEC1 in the epithelium of OSF promotes mesenchymal transition via activating FAK/Akt signal axis. J Oral Pathol Med 51:780-790 (2022). PubMed: 36053880
- Wang R et al. PLEKHH2 binds β-arrestin1 through its FERM domain, activates FAK/PI3K/AKT phosphorylation, and promotes the malignant phenotype of non-small cell lung cancer. Cell Death Dis 13:858 (2022). PubMed: 36209201
- Sasaki N et al. PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells. Front Cell Dev Biol 10:1003349 (2022). PubMed: 36313564
- Yang Y et al. Micro/Nanostructured Topography on Titanium Orchestrates Dendritic Cell Adhesion and Activation via β2 Integrin-FAK Signals. Int J Nanomedicine 17:5117-5136 (2022). PubMed: 36345509