Recombinant Anti-FANCI antibody [EPR23903-124] (ab245219)
![Western blot - Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-395068-anti-fanci-antibody-epr23903124-western-blot-hela.jpg "Western blot - Anti-FANCI antibody [EPR23903-124] (ab245219)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23903-124] to FANCI
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FANCI antibody [EPR23903-124]
See all FANCI primary antibodies -
Description
Rabbit monoclonal [EPR23903-124] to FANCI -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HeLa treated with 500ng/ml Mitomycin C for 6h, whole, Jurkat and Raji whole cell lysates; Human testis tissue lysate. IHC-P: Human testis, colon and tonsil tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa and Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23903-124 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/600.
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| IHC-P |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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| ICC/IF |
1/100.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/600. |
|
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
|
ICC/IF
1/100. |
Target
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Function
Required for maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination and in the repair of DNA cross-links. Participates in S phase and G2 phase checkpoint activation upon DNA damage. Promotes FANCD2 ubiquitination and recruitment to DNA repair sites. -
Involvement in disease
Defects in FANCI are a cause of Fanconi anemia complementation group I (FANCI) [MIM:609053]. It is a disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
Domain
The C-terminal 30 residues are probably required for function in DNA repair. -
Post-translational
modificationsMonoubiquitinated on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression.
Phosphorylated in response to DNA damage by ATM and/or ATR. -
Cellular localization
Nucleus. Concentrates in nuclear foci upon genotoxic stress. - Information by UniProt
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Database links
- Entrez Gene: 55215 Human
- Omim: 611360 Human
- SwissProt: Q9NVI1 Human
- Unigene: 513126 Human
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Alternative names
- FANCI antibody
- FANCI gene antibody
- FANCI_HUMAN antibody
see all
Images
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Western blot - Anti-FANCI antibody [EPR23903-124] (ab245219)All lanes : Anti-FANCI antibody [EPR23903-124] (ab245219) at 1/5000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Jurkat (human t cell leukemia T lymphocyte) whole cell lysate
Lane 3 : Raji ( human burkitts lymphoma B lymphocyte) whole cell lysate
Lane 4 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 26 seconds; Lane 4: 125 seconds.
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![Immunocytochemistry/ Immunofluorescence - Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-4-anti-fanci-antibody-epr23903124-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FANCI antibody [EPR23903-124] (ab245219)Immunofluorescent analysis of 100% methanol-fixed HeLa cells labelling FANCI with ab245219 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear foci staining in HeLa cell line, the signal increased a little after treatment with Mitomycin C (500ng/ml) for 6h. FANCI forms nuclear foci in cells in the absence or presence of exogenous DNA damage (PMID: 19561358). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-6-anti-fanci-antibody-epr23903124-flow-cytometry-jurkat.jpg)
Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] (ab245219)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling FANCI with ab245219 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-3-anti-fanci-antibody-epr23903124-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] (ab245219)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FANCI with ab245219 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human tonsil. The section was incubated with ab245219 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
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![Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-5-anti-fanci-antibody-epr23903124-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-FANCI antibody [EPR23903-124] (ab245219)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling FANCI with ab245219 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-2-anti-fanci-antibody-epr23903124-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] (ab245219)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FANCI with ab245219 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human colon. The section was incubated with ab245219 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
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![Western blot - Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-395067-anti-fanci-antibody-epr23903124-western-blot-untreated-hela.jpg)
Western blot - Anti-FANCI antibody [EPR23903-124] (ab245219)All lanes : Anti-FANCI antibody [EPR23903-124] (ab245219) at 1/2000 dilution
Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 500ng/ml Mitomycin C for 6h, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The more slowly migrating band is a monoubiquitinated form of FANCI, which can be induced by Mitomycin C treatment.
The expression profile observed is consistent with what has been described in the literature (PMID: 17460694).
Exposure time: 70 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-1-anti-fanci-antibody-epr23903124-immunohistochemistry-human-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCI antibody [EPR23903-124] (ab245219)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling FANCI with ab245219 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in human testis (PMID: 31219578). The section was incubated with ab245219 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
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![Anti-FANCI antibody [EPR23903-124] (ab245219)](https://www.abcam.com/ps/products/245/ab245219/Images/ab245219-9-benefits-of-recombinant-antibodies.png)
Anti-FANCI antibody [EPR23903-124] (ab245219)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab245219 has not yet been referenced specifically in any publications.