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    products/primary-antibodies/fatty-acid-synthase-antibody-epr7465-ab128856.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (12)

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Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
  • Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
  • Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 APC Carrier Free PE

You may also be interested in

Protein
Product image
Recombinant Human Fatty Acid Synthase protein (ab131929)
Primary
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Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174)
Assay
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Free Fatty Acid Assay Kit - Quantification (ab65341)

View more associated products

Overview

  • Product name

    Anti-Fatty Acid Synthase antibody [EPR7465]
    See all Fatty Acid Synthase primary antibodies
  • Description

    Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
    Database link: P49327

  • Positive control

    • WB: HeLa, HEK-293, 293T, A549, SHSY-5Y, NIH/3T3, L6 (Rat skeletal muscle myoblast) and MOLT4 cell lysates; Mouse brain, Rat brain lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cyt (intra): A549 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.58 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7465
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Synthesis
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Neuroscience
    • Neurology process
    • Metabolism
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Fatty Acid Synthase antibody [EPR7465] (ab204660)
    • PE Anti-Fatty Acid Synthase antibody [EPR7465] (ab223964)
    • APC Anti-Fatty Acid Synthase antibody [EPR7465] (ab223965)
    • Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Fatty Acid Synthase protein (ab131929)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 273 kDa.
ICC/IF (1)
1/50.

For unpurified version, use at 1/250 - 1/500 dilution.

Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 273 kDa.
ICC/IF
1/50.

For unpurified version, use at 1/250 - 1/500 dilution.

Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.
  • Tissue specificity

    Ubiquitous. Prominent expression in brain, lung, and liver.
  • Sequence similarities

    Contains 1 acyl carrier domain.
  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P49327 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2194 Human
    • Entrez Gene: 14104 Mouse
    • Entrez Gene: 50671 Rat
    • Omim: 600212 Human
    • SwissProt: P49327 Human
    • SwissProt: P19096 Mouse
    • SwissProt: P12785 Rat
    • Unigene: 83190 Human
    • Unigene: 236443 Mouse
    • Unigene: 9486 Rat
    see all
  • Alternative names

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

Images

  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
    Lane 5 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 6 : Mouse brain lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 8 : Rat brain lysate
    Lane 9 : L6 (Rat skeletal muscle myoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 273 kDa
    Observed band size: 273 kDa

  • Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (12)

Publishing research using ab128856? Please let us know so that we can cite the reference in this datasheet.

ab128856 has been referenced in 12 publications.

  • Zhang H  et al. Vitamin D receptor targets hepatocyte nuclear factor 4a and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem 295:3891-3905 (2020). PubMed: 32051143
  • Ma X  et al. Mechanism of continuous high temperature affecting growth performance, meat quality, and muscle biochemical properties of finishing pigs. Genes Nutr 14:23 (2019). PubMed: 31367261
  • Xue D  et al. Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss. Reprod Biol Endocrinol 17:52 (2019). PubMed: 31288842
  • Wu Y  et al. Berberine (BBR) Attenuated Palmitic Acid (PA)-Induced Lipotoxicity in Human HK-2 Cells by Promoting Peroxisome Proliferator-Activated Receptor a (PPAR-a). Med Sci Monit 25:7702-7708 (2019). PubMed: 31607744
  • Tao XR  et al. Angiotensinogen in hepatocytes contributes to Western diet-induced liver steatosis. J Lipid Res 60:1983-1995 (2019). PubMed: 31604805
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-6 of 6 Abreviews or Q&A

Flow Cytometry abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

Good
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Peripheral blood mononuclear cells)
Permeabilization
Yes - Methanol
Gating Strategy
Live cells.
Specification
Peripheral blood mononuclear cells
Fixation
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 19 2018

Western blot abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (rat liver)
Gel Running Conditions
Reduced Denaturing (7% SDS-PAGE)
Loading amount
20 µg
Specification
rat liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 05 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Cell (Tumor))
Permeabilization
Yes - 0.3% triton x100
Specification
Mouse Cell (Tumor)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 05 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Tissue sections (Peritoneal tumor)
Specification
Peritoneal tumor
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 14 2014

Western blot abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8% GEL, SDS PAGE)
Sample
Human Cell lysate - other (LS180)
Specification
LS180
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 01 2013

Question

Customer kindly contacted us regarding use of this product in Musca domestica.

Read More

Abcam community

Verified customer

Asked on Dec 05 2012

Answer

Thank you for contacting us.

I have reviewed the immunogen information for each of our anti-Fatty Acid Synthase antibodies. Unfortunately none have homologies which would guarantee they could work in Musca domestica, however ab128856 Rabbit monoclonal [EPR7465] to Fatty Acid Synthase does have an 87% positive identity score. Further the identical residues are grouped, which would be necessary for epitope recognition. Since the product has not yet been tested in house fly, we cannot guarantee that it will work, however, for UNTESTED applications; we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Dec 05 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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