Recombinant Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Fatty Acid Synthase antibody [EPR7465]
See all Fatty Acid Synthase primary antibodies -
Description
Rabbit monoclonal [EPR7465] to Fatty Acid Synthase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
Database link: P49327 -
Positive control
- WB: HeLa, HEK-293, 293T, A549, SHSY-5Y, NIH/3T3, L6 (Rat skeletal muscle myoblast) and MOLT4 cell lysates; Mouse brain, Rat brain lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cyt (intra): A549 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.58 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7465 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 273 kDa.
|
ICC/IF | (1) |
1/50.
For unpurified version, use at 1/250 - 1/500 dilution. |
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 273 kDa. |
ICC/IF
1/50. For unpurified version, use at 1/250 - 1/500 dilution. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. -
Tissue specificity
Ubiquitous. Prominent expression in brain, lung, and liver. -
Sequence similarities
Contains 1 acyl carrier domain. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Database links
- Entrez Gene: 2194 Human
- Entrez Gene: 14104 Mouse
- Entrez Gene: 50671 Rat
- Omim: 600212 Human
- SwissProt: P49327 Human
- SwissProt: P19096 Mouse
- SwissProt: P12785 Rat
- Unigene: 83190 Human
see all -
Alternative names
- [Acyl-carrier-protein] S acetyltransferase antibody
- [Acyl-carrier-protein] S malonyltransferase antibody
- 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
see all
Images
-
All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/5000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 4 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 5 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 6 : Mouse brain lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 8 : Rat brain lysate
Lane 9 : L6 (Rat skeletal muscle myoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 273 kDa
Observed band size: 273 kDa -
Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
Lane 3: A549 cell lysate (20 µg)
Lane 4: Hu liver tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (16)
ab128856 has been referenced in 16 publications.
- Gong TQ et al. Proteome-centric cross-omics characterization and integrated network analyses of triple-negative breast cancer. Cell Rep 38:110460 (2022). PubMed: 35235781
- Spohner AK et al. Mouse Liver Compensates Loss of Sgpl1 by Secretion of Sphingolipids into Blood and Bile. Int J Mol Sci 22:N/A (2021). PubMed: 34638955
- Carroll PA et al. The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis. PLoS Biol 19:e3001085 (2021). PubMed: 34669700
- Zuo Z et al. Integrated Analyses Identify Key Molecules and Reveal the Potential Mechanism of miR-182-5p/FOXO1 Axis in Alcoholic Liver Disease. Front Med (Lausanne) 8:767584 (2021). PubMed: 34950682
- Zhang H et al. Vitamin D receptor targets hepatocyte nuclear factor 4a and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem 295:3891-3905 (2020). PubMed: 32051143