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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)

Submit a review Submit a question References (1)
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
  • Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
  • Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7465] to Fatty Acid Synthase - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 APC PE Unconjugated

Overview

  • Product name

    Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free
    See all Fatty Acid Synthase primary antibodies

  • Description

    Rabbit monoclonal [EPR7465] to Fatty Acid Synthase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293, 293T, A549, SHSY-5Y, NIH/3T3, L6 (Rat skeletal muscle myoblast) and MOLT4 cell lysates; Mouse brain, Rat brain lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cyt (intra): A549 cells.

  • General notes

    ab227083 is the carrier-free version of ab128856.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.
WB
1/5000. Predicted molecular weight: 273 kDa.
ICC/IF
1/50.
Notes
Flow Cyt (Intra)
1/50.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.
WB
1/5000. Predicted molecular weight: 273 kDa.
ICC/IF
1/50.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

  • Tissue specificity

    Ubiquitous. Prominent expression in brain, lung, and liver.

  • Sequence similarities

    Contains 1 acyl carrier domain.

  • Cellular localization

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

Images

  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
    All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
    Lane 5 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 6 : Mouse brain lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 8 : Rat brain lysate
    Lane 9 : L6 (Rat skeletal muscle myoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 273 kDa
    Observed band size: 237 kDa why is the actual band size different from the predicted?



    This data was developed using ab128856, the same antibody clone in a different buffer formulation.

  • Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
    Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
    This data was developed using ab128856, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
    Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
    This data was developed using ab128856, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)

    This WB data was generated using the same anti-Fatty Acid Synthase antibody clone, EPR7465, in a different buffer formulation (cat# ab128856).

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg) 
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab128856 was shown to specifically react with Fatty Acid Synthase when Fatty Acid Synthase knockout samples were used. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
    Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab227083? Please let us know so that we can cite the reference in this datasheet.

ab227083 has been referenced in 1 publication.

  • Li M  et al. Caveolin1 protects against diet induced hepatic lipid accumulation in mice. PLoS One 12:e0178748 (2017). PubMed: 28570612

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