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    products/primary-antibodies/fbp1-antibody-epr4620-ab109732.pdf

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RecombinantRabMAb

Recombinant Anti-FBP1 antibody [EPR4620] (ab109732)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (14)

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Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)
  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
  • Flow Cytometry (Intracellular) - Anti-FBP1 antibody [EPR4620] (ab109732)
  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)
  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
  • Anti-FBP1 antibody [EPR4620] (ab109732)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4620] to FBP1
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-FBP1 antibody [EPR4620]
    See all FBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4620] to FBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human stomach, Human fetal liver, Human kidney, Mouse liver, Mouse kidney, Rat liver, and MCF7 lysates and DDDK-tagged FBP1 human full-length recombinant protein. ICC/IF: HeLa cell line Flow Cyt (intra): MCF7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4620
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-FBP1 antibody [EPR4620] - BSA and Azide free (ab240944)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MCF7 nuclear extract lysate (ab14860)
  • Recombinant Protein

    • Recombinant human FBP1 protein (Active) (ab208306)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/400.
ICC/IF
Use a concentration of 10 µg/ml.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
Notes
Flow Cyt (Intra)
1/400.
ICC/IF
Use a concentration of 10 µg/ml.
WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
Application notes
Is unsuitable for IHC-P.

Target

  • Pathway

    Carbohydrate biosynthesis; gluconeogenesis.
  • Involvement in disease

    Defects in FBP1 are the cause of fructose-1,6-bisphosphatase deficiency (FBPD) [MIM:229700]. FBPD is inherited as an autosomal recessive disorder mainly in the liver and causes life-threatening episodes of hypoglycemia and metabolic acidosis (lactacidemia) in newborn infants or young children.
  • Sequence similarities

    Belongs to the FBPase class 1 family.
  • Target information above from: UniProt accession P09467 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2203 Human
    • Entrez Gene: 14121 Mouse
    • Entrez Gene: 24362 Rat
    • Omim: 611570 Human
    • SwissProt: P09467 Human
    • SwissProt: Q9QXD6 Mouse
    • SwissProt: P19112 Rat
    • Unigene: 494496 Human
    • Unigene: 423078 Mouse
    • Unigene: 33703 Rat
    see all
  • Alternative names

    • 6-bisphosphatase 1 antibody
    • 6-bisphosphate 1-phosphohydrolase 1 antibody
    • D fructose 1 6 bisphosphate 1 phosphohydrolase 1 antibody
    • D-fructose-1 antibody
    • EC 3.1.3.11 antibody
    • F16P1_HUMAN antibody
    • FBP antibody
    • FBP 1 antibody
    • FBP1 antibody
    • FBPase 1 antibody
    • Fructose 1 6 bisphosphatase 1 antibody
    • Fructose bisphosphatase 1 antibody
    • Fructose-1 antibody
    • Growth inhibiting protein 17 antibody
    • Liver fructose bisphosphatase antibody
    see all

Images

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

    Lane 1 : DDDK-tagged FBP1 human full-length recombinant protein, 15 ng
    Lane 2 : DDDK-tagged FBP12 human full-length recombinant protein, 15 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa


    Exposure time: 5 seconds


    Blocking buffer and concentration: 5% NFDM/TBST. 

    Diluting buffer and concentration: 5% NFDM/TBST.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)
    Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1:50 dilution (7.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution (Purified)

    Lane 1 : Human kidney lysates
    Lane 2 : Mouse liver lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Rat liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Anti-FBP1 antibody [EPR4620] (ab109732) at 1/50000 dilution + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?

  • Flow Cytometry (Intracellular) - Anti-FBP1 antibody [EPR4620] (ab109732)
    Flow Cytometry (Intracellular) - Anti-FBP1 antibody [EPR4620] (ab109732)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FBP1 with purified ab109732 at 1/400 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

     

  • Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)
    Immunocytochemistry/ Immunofluorescence - Anti-FBP1 antibody [EPR4620] (ab109732)

    ICC/IF image of ab109732 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109732, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    Western blot - Anti-FBP1 antibody [EPR4620] (ab109732)
    All lanes : Anti-FBP1 antibody [EPR4620] (ab109732) at 1/1000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : MCF7 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 37 kDa

  • Anti-FBP1 antibody [EPR4620] (ab109732)
    Anti-FBP1 antibody [EPR4620] (ab109732)

Protocols

  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (14)

Publishing research using ab109732? Please let us know so that we can cite the reference in this datasheet.

ab109732 has been referenced in 14 publications.

  • Hu J  et al. Fructose-1,6-bisphosphatase aggravates oxidative stress-induced apoptosis in asthma by suppressing the Nrf2 pathway. J Cell Mol Med 25:5001-5014 (2021). PubMed: 33960626
  • Chen Z  et al. Andrographolide inhibits non-small cell lung cancer cell proliferation through the activation of the mitochondrial apoptosis pathway and by reprogramming host glucose metabolism. Ann Transl Med 9:1701 (2021). PubMed: 34988210
  • Han F  et al. Gastric cancer mesenchymal stem cells inhibit natural killer cell function by up-regulating FBP1. Cent Eur J Immunol 46:427-437 (2021). PubMed: 35125940
  • Yang C  et al. USP44 suppresses pancreatic cancer progression and overcomes gemcitabine resistance by deubiquitinating FBP1. Am J Cancer Res 9:1722-1733 (2019). PubMed: 31497353
  • Kanai S  et al. Phosphofructokinase-1 and fructose bisphosphatase-1 in canine liver and kidney. J Vet Med Sci 81:1515-1521 (2019). PubMed: 31474665
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-FBP1 antibody [EPR4620]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Jun 22 2017

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