Recombinant Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
![Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)](https://www.abcam.com/ps/products/137/ab137096/Images/ab137096-330548-anti-fetal-hemoglobin-antibody-epr9708b-western-blot.jpg "Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-fetal hemoglobin antibody [EPR9708(B)]
See all fetal hemoglobin primary antibodies -
Description
Rabbit monoclonal [EPR9708(B)] to fetal hemoglobin -
Host species
Rabbit -
Specificity
ab137096 recognises Hemoglobin gamma-1/2 chain. -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human fetal hemoglobin. The exact sequence is proprietary.
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Positive control
- WB: Human red blood cell and K562 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9708(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
Target
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Relevance
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3' -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3047 Human
- Entrez Gene: 3048 Human
- Omim: 142200 Human
- Omim: 142250 Human
- SwissProt: P69891 Human
- SwissProt: P69892 Human
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Alternative names
- A gamma globin antibody
- G gamma globin antibody
- Gamma 1 globin antibody
see all
Images
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Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)](https://www.abcam.com/ps/products/137/ab137096/Images/ab137096-264647-anti-fetal-hemoglobin-antibody-epr9708b-western-blot.jpg)
Western blot - Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)All lanes : Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096) at 1/2000 dilution (Unpurified)
Lane 1 : Human red blood cell
Lane 2 : K562 (human lymphoblast from chronic myelogenous leukemia) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution
Predicted band size: 16 kDaBlocking and Diluting Buffer:5% NFDM/TBST
Exposure time:3 min
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![Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)](https://www.abcam.com/ps/products/137/ab137096/Images/ab137096-4-benefits-of-recombinant-antibodies.png)
Anti-fetal hemoglobin antibody [EPR9708(B)] (ab137096)
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab137096 has been referenced in 6 publications.
- Jin T et al. Transmembrane Protein ANTXR1 Regulates γ-Globin Expression by Targeting the Wnt/β-Catenin Signaling Pathway. J Immunol Res 2022:8440422 (2022). PubMed: 35942209
- Li X et al. Transcriptional silencing of fetal hemoglobin expression by NonO. Nucleic Acids Res 49:9711-9723 (2021). PubMed: 34379783
- Boontanrart MY et al. ATF4 Regulates MYB to Increase ?-Globin in Response to Loss of ß-Globin. Cell Rep 32:107993 (2020). PubMed: 32755585
- Sun KT et al. Reciprocal regulation of ?-globin expression by exo-miRNAs: Relevance to ?-globin silencing in ß-thalassemia major. Sci Rep 7:202 (2017). WB . PubMed: 28303002
- Li J et al. SOX6 Downregulation Induces?-Globin in Humanß-Thalassemia Major Erythroid Cells. Biomed Res Int 2017:9496058 (2017). PubMed: 29333458
- Niu X et al. Combining Single Strand Oligodeoxynucleotides and CRISPR/Cas9 to Correct Gene Mutations in ß-Thalassemia-induced Pluripotent Stem Cells. J Biol Chem 291:16576-85 (2016). WB ; Human . PubMed: 27288406