Recombinant Anti-FGF2 antibody [EPR20145-219] (ab208687)
![Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-267923-anti-fgf2-antibody-epr20145-219-western-blot.jpg "Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20145-219] to FGF2
- Suitable for: WB, IP, ICC/IF, Indirect ELISA, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-FGF2 antibody [EPR20145-219]
See all FGF2 primary antibodies -
Description
Rabbit monoclonal [EPR20145-219] to FGF2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, Indirect ELISA, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant human FGF2 active protein (aa143-288); K562, U-87 MG and SK-OV-3 whole cell lysates; Human fetal kidney, prostate, fetal heart and testis lysates. ICC/IF: U-87 MG and SK-OV-3 cells. Flow Cyt (intra): K562 cells. IP: K562 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20145-219 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 24, 22, 18 kDa (predicted molecular weight: 30 kDa).
Application guaranteed in human only |
| IP |
1/30.
|
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| ICC/IF |
1/500.
|
|
| Indirect ELISA |
Use at an assay dependent concentration.
|
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| Flow Cyt (Intra) |
1/600.
Application guaranteed in human only |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 24, 22, 18 kDa (predicted molecular weight: 30 kDa). Application guaranteed in human only |
|
IP
1/30. |
|
ICC/IF
1/500. |
|
Indirect ELISA
Use at an assay dependent concentration. |
|
Flow Cyt (Intra)
1/600. Application guaranteed in human only |
Target
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Function
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). -
Tissue specificity
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Post-translational
modificationsPhosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
Cellular localization
Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. - Information by UniProt
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Database links
- Entrez Gene: 2247 Human
- Entrez Gene: 14173 Mouse
- Omim: 134920 Human
- SwissProt: P09038 Human
- SwissProt: P15655 Mouse
- Unigene: 284244 Human
- Unigene: 473689 Mouse
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Alternative names
- Basic fibroblast growth factor antibody
- Basic fibroblast growth factor bFGF antibody
- BFGF antibody
see all
Images
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Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)All lanes : Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 3 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 18,22,24 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 5 seconds.
The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).
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![Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-267921-anti-fgf2-antibody-epr20145-219-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and weak cytoplasmic staining on U-87 MG cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-267925-anti-fgf2-antibody-epr20145-219-western-blot.jpg)
Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/10000 dilution + Recombinant human FGF2 active protein (aa143-288) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-267922-anti-fgf2-antibody-epr20145-219-western-blot.jpg)
Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)All lanes : Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human prostate lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 18,22,24 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).
-
![Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-267920-anti-fgf2-antibody-epr20145-219-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear weak cytoplasmic staining on SK-OV-3 cell line.
The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-267919-anti-fgf2-antibody-epr20145-219-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-FGF2 antibody [EPR20145-219] (ab208687)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling FGF2 with ab208687 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-267918-anti-fgf2-antibody-epr20145-219-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] (ab208687)FGF2 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab208687 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab208687 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: K562 whole cell lysate 10µg (Input).
Lane 2: ab208687 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208687 in K562 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Indirect ELISA - Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-9-anti-fgf2-antibody-epr20145-219-elisa-mouse-fgf2.jpg)
Indirect ELISA - Anti-FGF2 antibody [EPR20145-219] (ab208687)ELISA analysis of Mouse FGF2 recombinant protein at 1000 ng/ml with ab208687. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Anti-FGF2 antibody [EPR20145-219] (ab208687)](https://www.abcam.com/ps/products/208/ab208687/Images/ab208687-8-benefits-of-recombinant-antibodies.png)
Anti-FGF2 antibody [EPR20145-219] (ab208687)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (9)
ab208687 has been referenced in 9 publications.
- Wang R et al. FGF2 Is Protective Towards Cisplatin-Induced KGN Cell Toxicity by Promoting FTO Expression and Autophagy. Front Endocrinol (Lausanne) 13:890623 (2022). PubMed: 35784556
- Huang D et al. Erythropoietin Activates Autophagy to Regulate Apoptosis and Angiogenesis of Periodontal Ligament Stem Cells via the Akt/ERK1/2/BAD Signaling Pathway under Inflammatory Microenvironment. Stem Cells Int 2022:9806887 (2022). PubMed: 36199627
- Shi X et al. Adipose-Derived Stromal Cell-Sheets Sandwiched, Book-Shaped Acellular Dermal Matrix Capable of Sustained Release of Basic Fibroblast Growth Factor Promote Diabetic Wound Healing. Front Cell Dev Biol 9:646967 (2021). PubMed: 33842472
- Zhu S et al. AAV2-mediated and hypoxia response element-directed expression of bFGF in neural stem cells showed therapeutic effects on spinal cord injury in rats. Cell Death Dis 12:274 (2021). PubMed: 33723238
- Li Z et al. Long non-coding RNA small nucleolar RNA host gene 1 knockdown suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating microRNA-424-5p/FGF2 in vitro. Exp Ther Med 21:325 (2021). PubMed: 33732298
- Huang X et al. MicroRNA-497-5p inhibits proliferation and invasion of non-small cell lung cancer by regulating FGF2. Oncol Lett 17:3425-3431 (2019). PubMed: 30867780
- Li C et al. MicroRNA-936 targets FGF2 to inhibit epithelial ovarian cancer aggressiveness by deactivating the PI3K/Akt pathway. Onco Targets Ther 12:5311-5322 (2019). PubMed: 31371979
- Zeng Z et al. Downregulation of CKS1B restrains the proliferation, migration, invasion and angiogenesis of retinoblastoma cells through the MEK/ERK signaling pathway. Int J Mol Med 44:103-114 (2019). PubMed: 31115482
- Duan H et al. LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299-3p/FGF2 axis. Life Sci 239:116856 (2019). PubMed: 31525429