Recombinant Anti-FGL1 antibody [EPR24018-27] (ab275091)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)](https://www.abcam.com/ps/products/275/ab275091/Images/ab275091-1-anti-fgl1-antibody-epr2401827-immunohistochemistry-human-liver.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24018-27] to FGL1
- Suitable for: IHC-P, IP, Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FGL1 antibody [EPR24018-27]
See all FGL1 primary antibodies -
Description
Rabbit monoclonal [EPR24018-27] to FGL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human liver tissue lysate: HepG2 and Huh7 whole cell lysates. IHC-P: Human liver and hepatocellular carcinoma tissue. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24018-27 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
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| Flow Cyt (Intra) |
1/50.
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| WB |
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 36 kDa).
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| Notes |
|---|
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/30. |
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Flow Cyt (Intra)
1/50. |
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WB
1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 36 kDa). |
Target
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Function
Has hepatocyte mitogenic activity. -
Tissue specificity
Liver specific. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2267 Human
- Omim: 605776 Human
- SwissProt: Q08830 Human
- Unigene: 491143 Human
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Alternative names
- FGL 1 antibody
- Fgl1 antibody
- FGL1_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FGL1 with ab275091 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human liver (PMID: 11470158). The section was incubated with ab275091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-FGL1 antibody [EPR24018-27] (ab275091)](https://www.abcam.com/ps/products/275/ab275091/Images/ab275091-7-anti-fgl1-antibody-epr2401827-western-blot-lysate.jpg)
Western blot - Anti-FGL1 antibody [EPR24018-27] (ab275091)All lanes : Anti-FGL1 antibody [EPR24018-27] (ab275091) at 1/1000 dilution
Lane 1 : Human liver tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 15 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 11004478).
Negative control: Human brain (PMID: 11470158).
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![Flow Cytometry (Intracellular) - Anti-FGL1 antibody [EPR24018-27] (ab275091)](https://www.abcam.com/ps/products/275/ab275091/Images/ab275091-4-anti-fgl1-antibody-epr2401827-flow-cytometry-hepg2.jpg)
Flow Cytometry (Intracellular) - Anti-FGL1 antibody [EPR24018-27] (ab275091)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling FGL1 with ab275091 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-FGL1 antibody [EPR24018-27] (ab275091)](https://www.abcam.com/ps/products/275/ab275091/Images/ab275091-5-anti-fgl1-antibody-epr2401827-immunoprecipitation-hepg2.jpg)
Immunoprecipitation - Anti-FGL1 antibody [EPR24018-27] (ab275091)FGL1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab275091 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275091 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab275091 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of abID1 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)](https://www.abcam.com/ps/products/275/ab275091/Images/ab275091-2-anti-fgl1-antibody-epr2401827-immunohistochemistry-human-hepatocellular-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling FGL1 with ab275091 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human hepatocellular carcinoma (PMID: 14981537). The section was incubated with ab275091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-FGL1 antibody [EPR24018-27] (ab275091)](https://www.abcam.com/ps/products/275/ab275091/Images/ab275091-6-anti-fgl1-antibody-epr2401827-western-blot-carcinoma.jpg)
Western blot - Anti-FGL1 antibody [EPR24018-27] (ab275091)Anti-FGL1 antibody [EPR24018-27] (ab275091) at 1/1000 dilution + Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)](https://www.abcam.com/ps/products/275/ab275091/Images/ab275091-3-anti-fgl1-antibody-epr2401827-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGL1 antibody [EPR24018-27] (ab275091)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling FGL1 with ab275091 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human cerebrum (PMID: 14981537). The section was incubated with ab275091 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Anti-FGL1 antibody [EPR24018-27] (ab275091)](https://www.abcam.com/ps/products/275/ab275091/Images/ab275091-8-benefits-of-recombinant-antibodies.png)
Anti-FGL1 antibody [EPR24018-27] (ab275091)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab275091 has not yet been referenced specifically in any publications.