Recombinant Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)
![Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)](https://www.abcam.com/ps/products/251/ab251378/Images/ab251378-411671-anti-fhl2-antibody-epr17860-20-bsa-and-azide-free-western-blot-u2-os-wt-u2-os-fhl2-knockout-hela-human-heart.jpg "Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17860-20] to FHL2 - BSA and Azide free
- Suitable for: WB, IP, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free
See all FHL2 primary antibodies -
Description
Rabbit monoclonal [EPR17860-20] to FHL2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U-2 OS, HeLa and K562 cell lysates.
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General notes
ab251378 is the carrier-free version of ab202584.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17860-20 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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| IP |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
|
IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. -
Tissue specificity
Expressed in skeletal muscle and heart. -
Sequence similarities
Contains 4 LIM zinc-binding domains. -
Domain
The third LIM zinc-binding mediates interaction with E4F1. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2274 Human
- Entrez Gene: 14200 Mouse
- Entrez Gene: 63839 Rat
- Omim: 602633 Human
- SwissProt: Q14192 Human
- SwissProt: O70433 Mouse
- SwissProt: O35115 Rat
- Unigene: 443687 Human
see all -
Alternative names
- AAG 11 antibody
- AAG11 antibody
- Aging associated gene 11 antibody
see all
Images
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Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)All lanes : Anti-FHL2 antibody [EPR17860-20] (ab202584) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : FHL2 knockout U-2 OS cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab202584).
Lanes 1 - 3: Merged signal (red and green). Green - ab202584 observed at 32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab202584 was shown to react with FHL2 in wild-type U-2 OS cells in western blot with loss of signal observed in FHL2 knockout sample. Wild-type and FHL2 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab202584 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)](https://www.abcam.com/ps/products/251/ab251378/Images/ab251378-387242-recombinant-anti-fhl2-antibody-epr17860-20-western-blot-hela-k562-lysates.jpg)
Western blot - Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)All lanes : Anti-FHL2 antibody [EPR17860-20] (ab202584) at 1/1000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : FHL2 knockout HeLa lysate
Lane 3 : K562 lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab202584).
Lanes 1-3: Merged signal (red and green). Green - ab202584 observed at 32 kDa. Red - loading control ab7291 observed at 50 kDa.
ab202584 Recombinant Anti-FHL2 antibody [EPR17860-20] was shown to specifically react with FHL2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265475 (knockout cell lysate ab257441) was used. Wild-type and FHL2 knockout samples were subjected to SDS-PAGE. ab202584 and Anti-alpha Tubulin antibody [DM1A] - Loading Control?(ab7291) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)](https://www.abcam.com/ps/products/251/ab251378/Images/ab251378-3-benefits-of-recombinant-antibodies.png)
Anti-FHL2 antibody [EPR17860-20] - BSA and Azide free (ab251378)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab251378 has not yet been referenced specifically in any publications.
