FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
Key features and details
- FITC Mouse monoclonal [1A4] to alpha smooth muscle Actin
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
FITC Anti-alpha smooth muscle Actin antibody [1A4]
See all alpha smooth muscle Actin primary antibodies -
Description
FITC Mouse monoclonal [1A4] to alpha smooth muscle Actin -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: SV40LT-SMC cells.
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General notes
This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species.
The antibody is conjugated with FITC at a ratio 6 Moles of Fluorescein to 1 Mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm, and maximum emission occurs at wavelenght 520 nm.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species. -
Clonality
Monoclonal -
Clone number
1A4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
- Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
- Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
- HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
- Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
- Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
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Isotype control
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KO cell lines
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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WB |
1/131.58. Predicted molecular weight: 42 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/131.58. Predicted molecular weight: 42 kDa. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 101093547 Cat
- Entrez Gene: 423787 Chicken
- Entrez Gene: 477587 Dog
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 81633 Rat
- Entrez Gene: 101121051 Sheep
- Entrez Gene: 322509 Zebrafish
see all -
Alternative names
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)ab8211 staining alpha smooth muscle Actin in wild-type HeLa cells (top panel) and ACTA2 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8211 at 5μg/ml concentration and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
ab8211 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8211 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 4% formaldehyde (10 min).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (64)
ab8211 has been referenced in 64 publications.
- Cui Y et al. Myogenic Vasoconstriction Requires Canonical Gq/11 Signaling of the Angiotensin II Type 1 Receptor. J Am Heart Assoc 11:e022070 (2022). PubMed: 35132870
- Tuca AC et al. Orchestrating the Dermal/Epidermal Tissue Ratio during Wound Healing by Controlling the Moisture Content. Biomedicines 10:N/A (2022). PubMed: 35740308
- Di Lorenzo A et al. Toll-like receptor 2 promotes breast cancer progression and resistance to chemotherapy. Oncoimmunology 11:2086752 (2022). PubMed: 35756841
- Ye Q et al. Understanding the development of oral epithelial organs through single cell transcriptomic analysis. Development 149:N/A (2022). PubMed: 35831953
- Teng YY et al. Dual-Action Icariin-Containing Thermosensitive Hydrogel for Wound Macrophage Polarization and Hair-Follicle Neogenesis. Front Bioeng Biotechnol 10:902894 (2022). PubMed: 35832407