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    products/primary-antibodies/fitc-alpha-smooth-muscle-actin-antibody-1a4-ab8211.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Validated using a knockout cell line

FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)

  • Datasheet
  • SDS
Reviews (9)Q&A (8)References (53)

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Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)

Key features and details

  • FITC Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG2a

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Carrier Free Unconjugated

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Overview

  • Product name

    FITC Anti-alpha smooth muscle Actin antibody [1A4]
    See all alpha smooth muscle Actin primary antibodies
  • Description

    FITC Mouse monoclonal [1A4] to alpha smooth muscle Actin
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: SV40LT-SMC cells.
  • General notes

    This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species.

    The antibody is conjugated with FITC at a ratio 6 Moles of Fluorescein to 1 Mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm, and maximum emission occurs at wavelenght 520 nm.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species.
  • Clonality

    Monoclonal
  • Clone number

    1A4
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Associated products

  • Alternative Versions

    • Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
    • Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
    • Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
    • HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
    • Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
    • Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
  • Isotype control

    • FITC Mouse IgG2a, kappa monoclonal [MOPC-173] - isotype control (ab18455)
  • KO cell lines

    • Human ACTA2 knockout HeLa cell line (ab264014)
  • Recombinant Protein

    • Recombinant Human alpha smooth muscle Actin protein (ab134555)
  • Related Products

    • Phalloidin-iFluor 488 Reagent (ab176753)
    • Phalloidin-iFluor 647 Reagent (ab176759)
    • Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
Use a concentration of 5 µg/ml.
WB
1/131.58. Predicted molecular weight: 42 kDa.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
1/131.58. Predicted molecular weight: 42 kDa.

Target

  • Function

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • Involvement in disease

    Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • Sequence similarities

    Belongs to the actin family.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P62736 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 101093547 Cat
    • Entrez Gene: 423787 Chicken
    • Entrez Gene: 477587 Dog
    • Entrez Gene: 59 Human
    • Entrez Gene: 11475 Mouse
    • Entrez Gene: 81633 Rat
    • Entrez Gene: 101121051 Sheep
    • Entrez Gene: 322509 Zebrafish
    • Omim: 102620 Human
    • SwissProt: P08023 Chicken
    • SwissProt: P62736 Human
    • SwissProt: P62737 Mouse
    • SwissProt: P62738 Rat
    • Unigene: 500483 Human
    • Unigene: 213025 Mouse
    • Unigene: 195319 Rat
    • Unigene: 3114 Rat
    see all
  • Alternative names

    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • alpha sma antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
    ab8211 staining alpha smooth muscle Actin in wild-type HeLa cells (top panel) and ACTA2 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8211 at 5μg/ml concentration and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
    Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)

    ab8211 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8211 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 4% formaldehyde (10 min).

Protocols

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (53)

Publishing research using ab8211? Please let us know so that we can cite the reference in this datasheet.

ab8211 has been referenced in 53 publications.

  • Jin X  et al. hsa_circNFXL1_009 modulates apoptosis, proliferation, migration, and potassium channel activation in pulmonary hypertension. Mol Ther Nucleic Acids 23:1007-1019 (2021). PubMed: 33614247
  • Li Z  et al. SCAP knockout in SM22a-Cre mice induces defective angiogenesis in the placental labyrinth. Biomed Pharmacother 133:111011 (2021). PubMed: 33227706
  • Yu J  et al. PLK1 Inhibition alleviates transplant-associated obliterative bronchiolitis by suppressing myofibroblast differentiation. Aging (Albany NY) 12:11636-11652 (2020). PubMed: 32541091
  • El-Kehdy H  et al. Inflammation Differentially Modulates the Biological Features of Adult Derived Human Liver Stem/Progenitor Cells. Cells 9:N/A (2020). PubMed: 32650454
  • Su X  et al. Effects of dynamic radial tensile stress on fibrocartilage differentiation of bone marrow mesenchymal stem cells. Biomed Eng Online 19:8 (2020). PubMed: 32024525
View all Publications for this product

Customer reviews and Q&As

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1-10 of 17 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 26 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Kidney H9 Organoid)
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney H9 Organoid
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 08 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 08 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 15 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Liver
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 24 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 24 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Breast
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 4°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. David Ivancic

Verified customer

Submitted Jul 18 2019

Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (Artery)
Permeabilization
Yes - 0.1% Triton X-100, 10 min
Specification
Artery
Blocking step
Serum as blocking agent for 4 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Chelsea Stowell

Verified customer

Submitted Mar 07 2018

Question

 Would you be able to provide our customer with a protocol for Flow Cytometry staining (which is best suited for your Smooth Muscle Actin Antibody, AB8211)? There are a number of protocols on the suggested website (https://www.abcam.com/index.html?pageconfig=resource&rid=11448) and would appreciate if you are able to advise which would best work with this antibody, AB8211.   With many thanks and best regards,,

Read More

Abcam community

Verified customer

Asked on Sep 23 2011

Answer

Thank you very much for your question. Indeed, the use of ab8211 in flow cytometry has been documented in the publication "Delayed Goblet Cell Hyperplasia, Acetylcholine Receptor Expression, and Worm Expulsion in SMC-Specific IL-4Rα–Deficient Mice " PMID 17222057 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1769405/?tool=pubmed "For the intracellular stain, cells in single cell suspension were incubated with 1% normal rat serum and stained with rat anti-mouse IL-4Rα–PE (mIL-4RM-1; BD Biosciences, http://www.bdbiosciences.com). Stained cells were then washed, fixed in 2% paraformaldehyde, permeabilized with saponin, preblocked with 2% NRS and Fc block (2.4G2), and stained with anti–α-actin FITC (Abcam, https://www.abcam.com) or isotype control IgG2a-FITC (BD Biosciences). " I hope this helps the customer. In conjunction with the protocol on intracellular flow cytometry https://www.abcam.com/index.html?pageconfig=resource&rid=11448, this should allow the reproduction of good results. Please let me know if the customer needs more information. Please let me also know if the customer has any problems. I would be pleased to investigate this for him. As you know, we do cover all our products to work as stated on the datasheet. I wish you a great week.

Read More

Abcam Scientific Support

Answered on Sep 23 2011

Question

 Thank you once again for your help extended. Our customer has reverted with the following question regarding the protocol referenced in your earlier email. It was mentioned:“Stained cells were then washed, fixed in 2% paraformaldehyde, permeabilized with saponin, preblocked with 2% NRS and Fc block (2.4G2), and stained with anti–α-actin FITC (Abcam, https://www.abcam.com) or isotype control IgG2a-FITC (BD Biosciences). " Would you be able to advise the purpose of pre-blocking with 2% NRS and Fc block? Is it a necessary step when using Ab8211?   With many thanks and best regards, 

Read More

Abcam community

Verified customer

Asked on Oct 07 2011

Answer

Thank you for your email. Indeed, I can confirm that the protocol is in reference to ab8211. The use of blocking is to decrease unspecific signal and background. The Fc block with the monoclonal antibody 2.4G2 is to block the Fc receptors expressed on certain cells, such as macrophages. The Fc receptors have a high affinity for immunoglobulins and might therefore bind the primary antibody via its Fc chain- resulting in an unspecific staining. The 2.4G2 is directed against Fc receptors and is shown to block their ability to bind immunoglobulins. The additional use of NRS (normal rat serum) is to block unspecific protein-protein interaction. It is therefore dependent on the cell type the customer is analysing whether these two blocking methods are necessary. It is however very good practice to include them and I can recommend to do so. I hope this information is helpful. Please do not hesitate to contact me again should the customer have other questions.

Read More

Abcam Scientific Support

Answered on Oct 07 2011

1-10 of 17 Abreviews or Q&A

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