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Anti-FITC antibody [F4/1] (ab112511)

Submit a review Q&A (3)References (3)
Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)
  • Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)
  • Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)

Key features and details

  • Mouse monoclonal [F4/1] to FITC
  • Suitable for: IP, IHC-Fr, Flow Cyt
  • Reacts with: Species independent
  • Isotype: IgG1

Overview

  • Product name

    Anti-FITC antibody [F4/1]
    See all FITC primary antibodies

  • Description

    Mouse monoclonal [F4/1] to FITC

  • Host species

    Mouse

  • Tested applications

    Suitable for: IP, IHC-Fr, Flow Cytmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    FITC conjugated to KLH

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    F4/1

  • Myeloma

    Sp2/0-Ag14

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab112511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

acetone-fixed
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

acetone-fixed
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

  • Alternative names

    • Fluorescein Isothiocyanate antibody

Images

  • Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)
    Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)

    Fluorescence quenching of FITC-labelled CD45 antibody using ab112511 at 10 µg/ml. Control IgG1

  • Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)
    Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)

    Fluorescence quenching of FITC-labelled B220 antibody using ab112511 at 10 µg/ml. Control IgG1

  • Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)
    Flow Cytometry - Anti-FITC antibody [F4/1] (ab112511)

    Fluorescence quenching of FITC-labelled CD8 antibody using ab112511 at 10 µg/ml. Control IgG1

References (3)

Publishing research using ab112511? Please let us know so that we can cite the reference in this datasheet.

ab112511 has been referenced in 3 publications.

  • Good Z  et al. Proliferation tracing with single-cell mass cytometry optimizes generation of stem cell memory-like T cells. Nat Biotechnol 37:259-266 (2019). PubMed: 30742126
  • Poeter M  et al. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63. Nat Commun 5:3738 (2014). PubMed: 24769558
  • Balogh P  et al. Hapten-mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody. J Immunol Methods 169:35-40 (1994). PubMed: 7510754

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Question

I was very concerned, but having received your message did spend several days of time and effort to test each of these antibodies. Unfortunately, none of the antibodies worked in IF. The high temperature and long transition time must have caused significant degradation. We order the vast majority of our antibodies from Abcam and have never had this problem before.

I need your assistance in arranging for a replacement of these antibodies a soon as possible. I am under considerable time pressure to complete a series of experiments involving these antibodies, which are critical for an important upcoming grant deadline. It was also a significant cost of about ˜$1,200.00

Please let me know what I can do to initiate this process. Is it possible to have the replacement antibodies shipped out to the lab? This would be a tremendous help!

Read More
Answer

Thank you for confirming these details. I am sorry to hear that these antibodies did not work in ICC/IF.
As requested, I have issued a free of charge replacement of ab3851, ab52060, and ab112511. These will be shipped out tonight for delivery tomorrow morning. It looks like that that original order was shipped on a Saturday for Monday's delivery. If you would prefer for us not to send orders on the weekend in the future, please let us know and we can make a note on your account.
To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Question

Will this antibody recognize an acetylated form of FITC.

Read More
Answer

Thank you for contacting Abcam.

I have been in contact with the lab and while we have not tested this clone directly with acetylated FITC, the antibody was tested with carboxy-fluorescein diacetate succinimydil ester (CFSE) in immunohistochemistry to trace CFSE-labeled lymphocytes in two-color staining (anti-FITC developed by PO-conjugated anti-mouse IgG) in combination with rat monoclonal antibodies developed with alkaline phosphatase. Since the acetyl groups are hydrolyzed within the cells after the uptake followed by the amino-reactive binding via the succinimidyl moiety, we most probably detect liberated (deacetylated) fluorescein, but have no direct data (by competition ELISA or anything) on whether plain FDA (fluorescein-diacetate) would be recognized by F4/1.

Please let me know if there is anything else I can help you with.

Read More

Question

since these antibodies I choose are FITC-conjugated, need I use F4/1(ab112511) as anti-FITC antibody for flowcytomety set? Hope to hear from you soon. Many thanks!

Read More
Answer

Thank you for contacting Abcam. I am replying to your question on behalf of my colleague Ada. The antibodies that have FITC conjugated to them do not need the anti-FITC antibody ab112511, if you simply want to detect FITC fluorescence by flow cytometry. If you have any questions or concerns, or if I have misunderstood your question, please reply.

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