Anti-FITC antibody [F4/1] (ab112511)
Key features and details
- Mouse monoclonal [F4/1] to FITC
- Suitable for: IP, IHC-Fr, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-FITC antibody [F4/1]
See all FITC primary antibodies -
Description
Mouse monoclonal [F4/1] to FITC -
Host species
Mouse -
Tested applications
Suitable for: IP, IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
FITC conjugated to KLH
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant -
Clonality
Monoclonal -
Clone number
F4/1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
acetone-fixed |
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. acetone-fixed |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. -
Alternative names
- Fluorescein Isothiocyanate antibody
Images
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Fluorescence quenching of FITC-labelled CD45 antibody using ab112511 at 10 µg/ml. Control IgG1
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Fluorescence quenching of FITC-labelled B220 antibody using ab112511 at 10 µg/ml. Control IgG1
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Fluorescence quenching of FITC-labelled CD8 antibody using ab112511 at 10 µg/ml. Control IgG1
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab112511 has been referenced in 3 publications.
- Good Z et al. Proliferation tracing with single-cell mass cytometry optimizes generation of stem cell memory-like T cells. Nat Biotechnol 37:259-266 (2019). PubMed: 30742126
- Poeter M et al. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63. Nat Commun 5:3738 (2014). PubMed: 24769558
- Balogh P et al. Hapten-mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody. J Immunol Methods 169:35-40 (1994). PubMed: 7510754