FITC Anti-CD14 antibody [MEM-15] (ab28061)
![Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)](https://www.abcam.com/ps/products/28/ab28061/Images/ab28061-284947-anti-cd14-antibody-mem-15-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)")
Key features and details
- FITC Mouse monoclonal [MEM-15] to CD14
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD14 antibody [MEM-15]
See all CD14 primary antibodies -
Description
FITC Mouse monoclonal [MEM-15] to CD14 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Ab28061 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. Ab28061 also recognises soluble forms of CD14 found in serum and in the urine of some nephrotic patients. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified): A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
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General notes
The antibody is free of unconjugated FITC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
High grade protease free BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP/Cy5.5® Anti-CD14 antibody [MEM-15], prediluted (ab157312)
- APC/Cy7® Anti-CD14 antibody [MEM-15], prediluted (ab194805)
- PE/Cy5® Anti-CD14 antibody [MEM-15] (ab232910)
- PE/Cy7® Anti-CD14 antibody [MEM-15] (ab234214)
- Alexa Fluor® 700 Anti-CD14 antibody [MEM-15] (ab269326)
- PerCP Anti-CD14 antibody [MEM-15] (ab269327)
- Biotin Anti-CD14 antibody [MEM-15] (ab28118)
- APC Anti-CD14 antibody [MEM-15] (ab60901)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
Use 20µl for 106 cells.
or 100 µl of whole blood. |
| Notes |
|---|
|
Flow Cyt
Use 20µl for 106 cells. or 100 µl of whole blood. |
Target
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Function
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
Tissue specificity
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
Sequence similarities
Contains 11 LRR (leucine-rich) repeats. -
Post-translational
modificationsN- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
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Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)
Flow cytometry analysis of human peripheral blood cells labelling CD14 with ab28061.
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![Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)](https://www.abcam.com/ps/products/28/ab28061/Images/ab28061-38961-anti-cd14-antibody-mem-15-fitc-flow-cytometry.jpg)
Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (ab28061)This image is courtesy of an anonymous Abreviewab28061 staining CD14 in peripheral blood mononuclear cells by flow cytometry. The fibroblasts were prepared using 10 uL FITC antiCD14 to 50 uL (~100,000) cells in PBS/5%FCS and incubated on ice for 45 min. Cells were gated on viable cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab28061 has been referenced in 7 publications.
- Meng Q et al. Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway. J Cell Mol Med 25:1867-1883 (2021). PubMed: 33417281
- Meng Q & Qiu B Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression. Front Physiol 11:441 (2020). PubMed: 32528301
- Gonda K et al. Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer. Oncol Lett 14:1766-1774 (2017). PubMed: 28789407
- Shi Q et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). PubMed: 29096715
- Jensen J et al. Functionalization of polycaprolactone scaffolds with hyaluronic acid and ß-TCP facilitates migration and osteogenic differentiation of human dental pulp stem cells in vitro. Tissue Eng Part A 21:729-39 (2015). PubMed: 25252795
- Steensma MR et al. Targeting the giant cell tumor stromal cell: functional characterization and a novel therapeutic strategy. PLoS One 8:e69101 (2013). Flow Cyt ; Human . PubMed: 23922683
- Sing A et al. Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med 196:1017-24 (2002). PubMed: 12391013