FITC Anti-CD4 antibody [MEM-241] (ab18281)
![Flow Cytometry - FITC Anti-CD4 antibody [MEM-241] (ab18281)](https://www.abcam.com/ps/products/18/ab18281/Images/ab18281-27927-anti-cd4-antibody-mem-241-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD4 antibody [MEM-241] (ab18281)")
Key features and details
- FITC Mouse monoclonal [MEM-241] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Overview
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Product name
FITC Anti-CD4 antibody [MEM-241]
See all CD4 primary antibodies -
Description
FITC Mouse monoclonal [MEM-241] to CD4 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein (Human).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MEM-241 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- APC Anti-CD4 antibody [MEM-241] (ab18280)
- PE Anti-CD4 antibody [MEM-241] (ab18282)
- APC/Cy7® Anti-CD4 antibody [MEM241] (ab233298)
- PE/Cy7® Anti-CD4 antibody [MEM241] (ab233660)
- Alexa Fluor® 700 Anti-CD4 antibody [MEM-241] (ab270671)
- Biotin Anti-CD4 antibody [MEM-241] (ab28069)
- PerCP Anti-CD4 antibody [MEM-241] (ab65951)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 4.15µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use 4.15µl for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - FITC Anti-CD4 antibody [MEM-241] (ab18281)Overlay histogram showing peripheral blood lymphocytes stained with ab18281 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18281, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab18281 has been referenced in 2 publications.
- Cui C et al. Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor. BMC Immunol 23:58 (2022). PubMed: 36434505
- Eckert AF et al. Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy. Elife 9:N/A (2020). PubMed: 32441251