FITC Anti-Ctip2 antibody [25B6] (ab123449)
![Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449)](https://www.abcam.com/ps/products/123/ab123449/Images/ab123449-477117-Project20210823-ab123449-AP6482601-5ug-Jurkat-MeOH-DS-image-JPEG.jpg "Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449)")
Key features and details
- FITC Rat monoclonal [25B6] to Ctip2
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
FITC Anti-Ctip2 antibody [25B6]
See all Ctip2 primary antibodies -
Description
FITC Rat monoclonal [25B6] to Ctip2 -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human Ctip2 aa 1-150.
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Positive control
- Jurkat cells.
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General notes
Fluorochrome/Protein Ratio: at least 3 moles FITC per mole of IgG.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab123449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
25B6 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 0.125 - 1 µg/ml.
|
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| Flow Cyt | (1) |
Use a concentration of 0.125 - 1 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 0.125 - 1 µg/ml. |
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Flow Cyt
Use a concentration of 0.125 - 1 µg/ml. |
Target
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Function
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
Tissue specificity
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
Sequence similarities
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 64919 Human
- Entrez Gene: 58208 Mouse
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- SwissProt: Q99PV8 Mouse
- Unigene: 709690 Human
- Unigene: 392694 Mouse
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Alternative names
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449)
ab123449 staining CTIP2 in Jurkat cells. The cells were fixed with 80% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab123449 at 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry - FITC Anti-Ctip2 antibody [25B6] (ab123449)](https://www.abcam.com/ps/products/123/ab123449/Images/ab123449-136695-anti-ctip2-antibody-25b6-fitc-flow-cytometry.gif)
Flow Cytometry - FITC Anti-Ctip2 antibody [25B6] (ab123449)ab123449 at 0.125µg/ml staining Ctip2 in Jurkat cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block.The Jurkat cells show a clear CTIP2 specific signal -
![Flow Cytometry - FITC Anti-Ctip2 antibody [25B6] (ab123449)](https://www.abcam.com/ps/products/123/ab123449/Images/ab123449-136694-anti-ctip2-antibody-25b6-fitc-flow-cytometry.gif)
Flow Cytometry - FITC Anti-Ctip2 antibody [25B6] (ab123449)ab123449 at 0.125µg/ml staining Ctip2 in SH-SY5Y cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block. The SH-SY5Y cells do not show significant CTIP2 signal over the negative control signal.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab123449 has been referenced in 3 publications.
- Del Toro D et al. Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons. Cell 180:323-339.e19 (2020). PubMed: 31928845
- Zhong C et al. Differential Expression of the Transcription Factor GATA3 Specifies Lineage and Functions of Innate Lymphoid Cells. Immunity 52:83-95.e4 (2020). PubMed: 31882362
- Forouzanfar F et al. HIV-1 Vpr mediates the depletion of the cellular repressor CTIP2 to counteract viral gene silencing. Sci Rep 9:13154 (2019). PubMed: 31511615
