Recombinant Anti-FKBP52 antibody [EPR6618] (ab129097)
![Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.com/ps/products/129/ab129097/Images/ab129097-143074-anti-fkbp52-antibody-epr6618-western-blot.jpg "Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6618] to FKBP52
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FKBP52 antibody [EPR6618]
See all FKBP52 primary antibodies -
Description
Rabbit monoclonal [EPR6618] to FKBP52 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human FKBP52 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, Jurkat, K562, and MCF7 cell lysates; Human colon and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6618 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
Target
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Function
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
Domain
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
Post-translational
modificationsPhosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 2288 Human
- Entrez Gene: 14228 Mouse
- Entrez Gene: 260321 Rat
- Omim: 600611 Human
- SwissProt: Q02790 Human
- SwissProt: P30416 Mouse
- SwissProt: Q9QVC8 Rat
- Unigene: 524183 Human
see all -
Alternative names
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
Images
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Western blot - Anti-FKBP52 antibody [EPR6618] (ab129097)All lanes : Anti-FKBP52 antibody [EPR6618] (ab129097) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : K562 cell lysates
Lane 4 : MCF7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.com/ps/products/129/ab129097/Images/ab129097-263733-anti-fkbp52-antibody-epr6618-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [EPR6618] (ab129097)ab129097 staining FKBP52 in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.com/ps/products/129/ab129097/Images/ab129097-143073-anti-fkbp52-antibody-epr6618-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.com/ps/products/129/ab129097/Images/ab129097-143072-anti-fkbp52-antibody-epr6618-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6618] (ab129097)ab129097, at a 1/250 dilution, staining FKBP52 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.com/ps/products/129/ab129097/Images/ab129097-143071-anti-fkbp52-antibody-epr6618.jpg)
OI-RD Scanning - Anti-FKBP52 antibody [EPR6618] (ab129097) -
![Anti-FKBP52 antibody [EPR6618] (ab129097)](https://www.abcam.com/ps/products/129/ab129097/Images/ab129097-6-benefits-of-recombinant-antibodies.png)
Anti-FKBP52 antibody [EPR6618] (ab129097)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab129097 has been referenced in 7 publications.
- Lin CC et al. Effects of RU486 in Treatment of Traumatic Stress-Induced Glucocorticoid Dysregulation and Fear-Related Abnormalities: Early versus Late Intervention. Int J Mol Sci 23:N/A (2022). PubMed: 35628305
- Lin CC et al. Effects of early life social experience on fear extinction and related glucocorticoid profiles - behavioral and neurochemical approaches in a rat model of PTSD. Behav Brain Res 391:112686 (2020). PubMed: 32428628
- Xiao K et al. HuR Affects Proliferation and Apoptosis of Chronic Lymphocytic Leukemia Cells via NF-?B Pathway. Biomed Res Int 2020:1481572 (2020). PubMed: 32908868
- Song S & Tan Y Expression of FKBP52 in the ovaries of PCOS rats. Int J Mol Med 43:868-878 (2019). PubMed: 30483787
- Li YE et al. Identification of high-confidence RNA regulatory elements by combinatorial classification of RNA-protein binding sites. Genome Biol 18:169 (2017). PubMed: 28886744
- Cao G et al. Xiao Yao San against Corticosterone-Induced Stress Injury via Upregulating Glucocorticoid Receptor Reaction Element Transcriptional Activity. Evid Based Complement Alternat Med 2016:5850739 (2016). PubMed: 27822288
- Caldwell KK et al. The impact of prenatal alcohol exposure on hippocampal-dependent outcome measures is influenced by prenatal and early-life rearing conditions. Alcohol Clin Exp Res 39:631-9 (2015). PubMed: 25752869