Recombinant Anti-FKBP52 antibody [EPR6619] (ab124906)
![Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.com/ps/products/124/ab124906/Images/ab124906-138889-anti-fkbp52-antibody-epr6619-western-blot.jpg "Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6619] to FKBP52
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FKBP52 antibody [EPR6619]
See all FKBP52 primary antibodies -
Description
Rabbit monoclonal [EPR6619] to FKBP52 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human FKBP52 aa 400-500 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, MCF7, Jurkat and K562 cell lysates; Human breast tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.38 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6619 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/50000 - 1/200000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Application notes
Is unsuitable for Flow Cyt (Intra) or ICC/IF.
Target
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Function
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
Domain
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
Post-translational
modificationsPhosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 2288 Human
- Omim: 600611 Human
- SwissProt: Q02790 Human
- Unigene: 524183 Human
- Unigene: 713721 Human
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Alternative names
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
Images
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Western blot - Anti-FKBP52 antibody [EPR6619] (ab124906)All lanes : Anti-FKBP52 antibody [EPR6619] (ab124906) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.com/ps/products/124/ab124906/Images/ab124906-138888-anti-fkbp52-antibody-epr6619-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [EPR6619] (ab124906)ab124906, at 1/100 dilution, staining FKBP52 in paraffin-embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.com/ps/products/124/ab124906/Images/ab124906-138887-anti-fkbp52-antibody-epr6619.jpg)
OI-RD Scanning - Anti-FKBP52 antibody [EPR6619] (ab124906) -
![Anti-FKBP52 antibody [EPR6619] (ab124906)](https://www.abcam.com/ps/products/124/ab124906/Images/ab124906-4-benefits-of-recombinant-antibodies.png)
Anti-FKBP52 antibody [EPR6619] (ab124906)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124906 has been referenced in 1 publication.
- Maeda K et al. FKBP51 and FKBP52 regulate androgen receptor dimerization and proliferation in prostate cancer cells. Mol Oncol 16:940-956 (2022). PubMed: 34057812