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Anti-FKBP52 antibody [Hi52C] (ab59460)

Submit a review Q&A (1)References (3)
Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [Hi52C] (ab59460)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [Hi52C] (ab59460)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [Hi52C] (ab59460)
  • Flow Cytometry - Anti-FKBP52 antibody [Hi52C] (ab59460)

Key features and details

  • Mouse monoclonal [Hi52C] to FKBP52
  • Suitable for: Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-FKBP52 antibody [Hi52C]
    See all FKBP52 primary antibodies

  • Description

    Mouse monoclonal [Hi52C] to FKBP52

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide corresponding to human FKBP52

  • Positive control

    • IHC-P: Human prostate tissue; Mouse backskin tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab59460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 10 µg/ml.
Notes
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Function

    Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.

  • Tissue specificity

    Widely expressed.

  • Sequence similarities

    Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.

  • Domain

    The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.

  • Post-translational
    modifications

    Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • 51 kDa FK506-binding protein antibody
      • 52 kDa FK506 binding protein antibody
      • 52 kDa FK506-binding protein antibody
      • 52 kDa FKBP antibody
      • 59 kDa immunophilin antibody
      • FK506 binding protein 4 antibody
      • FK506-binding protein 4 antibody
      • FKBP 4 antibody
      • FKBP 52 antibody
      • FKBP 59 antibody
      • FKBP-4 antibody
      • FKBP-52 antibody
      • FKBP4 antibody
      • FKBP4_HUMAN antibody
      • FKBP51 antibody
      • FKBP52 protein antibody
      • FKBP59 antibody
      • HBI antibody
      • Hsp 56 antibody
      • HSP binding immunophilin antibody
      • HSP-binding immunophilin antibody
      • Hsp56 antibody
      • Immunophilin FKBP52 antibody
      • N-terminally processed antibody
      • p52 antibody
      • p59 antibody
      • p59 protein antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • peptidyl-prolyl cis-trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
      • Peptidylprolyl cis trans isomerase antibody
      • PPIase antibody
      • PPIase FKBP4 antibody
      • Rotamase antibody
      • T cell FK506 binding protein 59kD antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [Hi52C] (ab59460)
      Immunocytochemistry/ Immunofluorescence - Anti-FKBP52 antibody [Hi52C] (ab59460)
      ICC/IF image of ab59460 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59460, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [Hi52C] (ab59460)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [Hi52C] (ab59460)

      Paraffin-embedded mouse backskin tissue stained for FKBP52 using ab59460 at 1/100 dilution in immunohistochemical analysis.

      Fixation: Bouin's Fixative. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 dilution for 1 hour at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [Hi52C] (ab59460)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FKBP52 antibody [Hi52C] (ab59460)

      ab59460 at 1/100 dilution staining FKBP52 in prostate tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A HRP conjugated goat anti mouse secondary was used at 1/10 dilution.

    • Flow Cytometry - Anti-FKBP52 antibody [Hi52C] (ab59460)
      Flow Cytometry - Anti-FKBP52 antibody [Hi52C] (ab59460)
      Overlay histogram showing HeLa cells stained with ab59460 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59460, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References (3)

    Publishing research using ab59460? Please let us know so that we can cite the reference in this datasheet.

    ab59460 has been referenced in 3 publications.

    • Hong Y  et al. Amelioration of muscle wasting by glucagon-like peptide-1 receptor agonist in muscle atrophy. J Cachexia Sarcopenia Muscle N/A:N/A (2019). PubMed: 31020810
    • Harrell CS  et al. Pharmacological stimulation of hypoxia inducible factor-1a facilitates the corticosterone response to a mild acute stressor. Neurosci Lett 600:75-9 (2015). PubMed: 26037418
    • Pare JM  et al. Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells. Mol Biol Cell 24:2303-10 (2013). PubMed: 23741051

    Customer reviews and Q&As

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    Question

    No bands in Western blot using these antibodies.

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    Answer

    Thank you very much for your call today and for letting us know about the trouble with these antibodies.

    I'msending a free of charge vial of ab3341 on the order *** which should arrive tomorrow, andI'm also sending a vial of ab129097 on the order *** which we expect to arrive around Thursday of next week. Please keep me updated about how these replacement antibodies work for you.

    As we discussed, since ab11132 has not been tested in Western blot in our labs, we can not guarantee that it works in Western blot. So, I have created the discount code below, which can be redeemed for a free primary antibody after submitting an Abreview of ab11132 in Western blot:

    DISCOUNT CODE: ***
    Expiration date: November 26, 2012

    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab11132 in Western blotand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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