Anti-FKLF / KLF11 antibody [OTI4H7] (ab279389)
Key features and details
- Mouse monoclonal [OTI4H7] to FKLF / KLF11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-FKLF / KLF11 antibody [OTI4H7]
See all FKLF / KLF11 primary antibodies -
Description
Mouse monoclonal [OTI4H7] to FKLF / KLF11 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human FKLF/ KLF11 aa 1-274. NP_003588
Database link: O14901 -
Positive control
- WB: HEK-293T cell extract. HEK-293T cells transfected with pCMV6-ENTRY FKLF / KLF11 cDNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OTI4H7 -
Isotype
IgG1 -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab279389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 55 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 55 kDa. |
Target
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Function
Transcription factor. Activates the epsilon- and gamma-globin gene promoters and, to a much lower degree, the beta-globin gene and represses promoters containing SP1-like binding inhibiting cell growth. Represses transcription of SMAD7 which enhances TGF-beta signaling. Induces apoptosis. -
Tissue specificity
Ubiquitous. Higher expression in erythroid cells. -
Involvement in disease
Defects in KLF11 are the cause of maturity-onset diabetes of the young type 7 (MODY7) [MIM:610508]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8462 Human
- Omim: 603301 Human
- SwissProt: O14901 Human
- Unigene: 12229 Human
- Unigene: 694968 Human
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Alternative names
- 9830142A17 antibody
- D12Ertd427e antibody
- FKLF antibody
see all
Images
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Anti-FKLF / KLF11 antibody [OTI4H7] (ab279389) at 1/500 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell extract at 35 µg
Predicted band size: 55 kDa -
All lanes : Anti-FKLF / KLF11 antibody [OTI4H7] (ab279389) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with the pCMV6-ENTRY control
Lane 2 : HEK-293T cells pCMV6-ENTRY FKLF / KLF11 cDNA for 48 hrs and lysed
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab279389 has been referenced in 1 publication.
- Li J et al. KLF11 promotes the progression of glioma via regulating Holliday junction recognition protein. Cell Biol Int 46:1144-1155 (2022). PubMed: 35293659