Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
![Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)](https://www.abcam.com/ps/products/214/ab214335/Images/ab214335-267151-anti-flavivirus-e-glycoprotein-antibody-hf8-1-c1-6-e12-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)")
Key features and details
- Mouse monoclonal [HF8-1/C1-6/E12] to Flavivirus E-glycoprotein
- Suitable for: WB, ICC/IF, ELISA, Sandwich ELISA
- Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus
- Isotype: IgG1
Overview
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Product name
Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12]
See all Flavivirus E-glycoprotein primary antibodies -
Description
Mouse monoclonal [HF8-1/C1-6/E12] to Flavivirus E-glycoprotein -
Host species
Mouse -
Specificity
ab214335 reacts with a conformational epitope present in DENV1, 2, 3, 4, ZIKV, JEV and WNV. -
Tested applications
Suitable for: WB, ICC/IF, ELISA, Sandwich ELISAmore details -
Species reactivity
Reacts with: Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4, Japanese encephalitis virus, West Nile virus, Zika virus -
Immunogen
Recombinant full length protein corresponding to Dengue virus 1 Flavivirus E-glycoprotein aa 250-800.
Database link: P27909 -
Positive control
- WB: DENV1 (Hawaii), DENV2 (NGC), DENV3 (H89), DENV4 (H241), ZIKV (Cambodia), JEV (Nakayama), WNV (E101) infected C6/36 cell lysates; ICC/IF: DENV1, DENV2, DENV3, DENV4, ZIKV infected cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Proclin 150
Constituents: 10% Fetal Bovine Serum (FBS), 89.9% RPMI 1640 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
HF8-1/C1-6/E12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214335 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
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| ICC/IF |
Use at an assay dependent concentration.
Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
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| ELISA |
Use at an assay dependent concentration.
This monoclonal antibody has been primarily used for the capture/detection of DENV1, 2, 3, 4, ZIKV (data not shown), JEV and WNV antigens in an antigen capture ELISA. |
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| Sandwich ELISA |
Use at an assay dependent concentration.
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| Notes |
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WB
Use at an assay dependent concentration. Detects DENV1, 2, 3, 4, ZIKV, JEV and WNV by western blot (non-reducing conditions). |
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ICC/IF
Use at an assay dependent concentration. Detects DENV1, 2, 4 and ZIKV in immunofluorescence DENV3 and 4 foci in FRNT. Not tested against ZIKV, JEV or WNV for foci staining in FRNT. Can neutralize DENV2, 3, 4 and ZIKV in a standard VERO PRNT. Not tested against JEV and WNV by PRNT. |
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ELISA
Use at an assay dependent concentration. This monoclonal antibody has been primarily used for the capture/detection of DENV1, 2, 3, 4, ZIKV (data not shown), JEV and WNV antigens in an antigen capture ELISA. |
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Sandwich ELISA
Use at an assay dependent concentration. |
Target
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Relevance
The Flaviviridae are a family of viruses that are primarily spread through arthropod vectors (mainly ticks and mosquitoes). Flaviviridae have monopartite, linear, single-stranded RNA genomes of positive polarity, 9.6- to 12.3-kilobase in length with enveloped and spherical virus particles, about 40-60 nm in diameter. -
Database links
- SwissProt: P27909 Dengue virus 1
- SwissProt: P29990 Dengue virus 2
- SwissProt: P29991 Dengue virus 2
- SwissProt: Q6YMS4 Dengue virus 3
- SwissProt: Q2YHF0 Dengue virus 4
- SwissProt: P14403 Japanese encephalitis virus
- SwissProt: P06935 West Nile virus
- SwissProt: Q32ZE1 Zika virus
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Alternative names
- Flavivirus envelope glycoprotein antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
Immunofluorescent analysis of DENV1, DENV2, DENV3, DENV4 and ZIKV infected cells labeling Flavivirus E-glycoprotein using ab214335.
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![ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)](https://www.abcam.com/ps/products/214/ab214335/Images/ab214335-267185-anti-flavivirus-e-glycoprotein-antibody-hf8-1-c1-6-e12-elisa.jpg)
ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)ELISA analysis using Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
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![Sandwich ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)](https://www.abcam.com/ps/products/214/ab214335/Images/ab214335-267184-anti-flavivirus-e-glycoprotein-antibody-hf8-1-c1-6-e12-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)Sandwich ELISA detection Flavivirus E-glycoprotein in Zika infected and non infected C6/36 cells using ab214335 and other anti Flavivirus E-glycoprotein antibodies as capture antibody.
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![Western blot - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)](https://www.abcam.com/ps/products/214/ab214335/Images/ab214335-267152-anti-flavivirus-e-glycoprotein-antibody-hf8-1-c1-6-e12-western-blot.jpg)
Western blot - Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)All lanes : Anti-Flavivirus E-glycoprotein antibody [HF8-1/C1-6/E12] (ab214335)
Lane 1 : DENV1 (Hawaii) infected C6/36 cell lysate
Lane 2 : DENV2 (NGC) infected C6/36 cell lysate
Lane 3 : DENV3 (H89) infected C6/36 cell lysate
Lane 4 : DENV4 (H241) infected C6/36 cell lysate
Lane 5 : ZIKV (Cambodia) infected C6/36 cell lysate
Lane 6 : JEV (Nakayama) infected C6/36 cell lysate
Lane 7 : YFV (17D) infected C6/36 cell lysate
Lane 8 : WNV (E101) infected C6/36 cell lysate
Lane 9 : C6/36 cell lysate control
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab214335 has not yet been referenced specifically in any publications.