Anti-FLG2 antibody (ab122011)
Key features and details
- Rabbit polyclonal to FLG2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FLG2 antibody -
Description
Rabbit polyclonal to FLG2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
amino acids 280-381 of Human FLG2 (Q5D862).
-
Positive control
- Human prostate tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
|
| IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
-
Sequence similarities
Belongs to the S100-fused protein family.
In the N-terminal section; belongs to the S-100 family.
Contains 2 EF-hand domains.
Contains 10 filaggrin repeats. - Information by UniProt
-
Database links
- Entrez Gene: 388698 Human
- SwissProt: Q5D862 Human
- Unigene: 156124 Human
-
Alternative names
- FILA2_HUMAN antibody
- Filaggrin-2 antibody
- FLG-2 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-FLG2 antibody (ab122011)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122011 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLG2 antibody (ab122011)ab122011, staining FLG2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab122011 has been referenced in 1 publication.
- Meisser SS et al. Skin barrier damage after exposure to paraphenylenediamine. J Allergy Clin Immunol 145:619-631.e2 (2020). PubMed: 31783056