Anti-FOXA1 antibody [1B1] (ab55178)
![Western blot - Anti-FOXA1 antibody [1B1] (ab55178)](https://www.abcam.com/ps/products/55/ab55178/Images/ab55178-448911-anti-foxa1-antibody-western-blot-hela-hap1-lysates.jpg "Western blot - Anti-FOXA1 antibody [1B1] (ab55178)")
Key features and details
- Mouse monoclonal [1B1] to FOXA1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-FOXA1 antibody [1B1]
See all FOXA1 primary antibodies -
Description
Mouse monoclonal [1B1] to FOXA1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human FOXA1 aa 367-472.
Database link: P55317 -
Positive control
- WB: HepG2 and HeLa cell lysates.
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General notes
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
1B1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis. -
Tissue specificity
Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3169 Human
- Omim: 602294 Human
- SwissProt: P55317 Human
- Unigene: 163484 Human
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Alternative names
- forkhead box A1 antibody
- Forkhead box protein A1 antibody
- FOX A1 antibody
see all
Images
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Western blot - Anti-FOXA1 antibody [1B1] (ab55178)All lanes : Anti-FOXA1 antibody [1B1] (ab55178) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FOXA1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDaLanes 1- 2: Merged signal (red and green). Green - ab55178 observed at 49 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.
ab55178 was shown to react with FOXA1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261823 (knockout cell lysate ab256920) was used. Wild-type HeLa and FOXA1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab55178 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [1B1] (ab55178)](https://www.abcam.com/ps/products/55/ab55178/Images/ab55178-68368-anti-foxa1-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA1 antibody [1B1] (ab55178)FOXA1 antibody (ab55178) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human salivary gland.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [1B1] (ab55178)](https://www.abcam.com/ps/products/55/ab55178/Images/ab55178-68366-anti-foxa1-antibody-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FOXA1 antibody [1B1] (ab55178)ab55178 at 10 ug/ml staining FOXA1 in human HepG2 cells by Immunocytochemistry/ Immunofluorescence.
This image was generated using the ascites version of the product.
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![Western blot - Anti-FOXA1 antibody [1B1] (ab55178)](https://www.abcam.com/ps/products/55/ab55178/Images/ab55178-68367-anti-foxa1-antibody-western-blot.jpg)
Western blot - Anti-FOXA1 antibody [1B1] (ab55178)FOXA1 antibody (ab55178) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-FOXA1 antibody [1B1] (ab55178)](https://www.abcam.com/ps/products/55/ab55178/Images/FOXA1-Primary-antibodies-ab55178-2.jpg)
Flow Cytometry - Anti-FOXA1 antibody [1B1] (ab55178)Overlay histogram showing HepG2 cells stained with ab55178 (red line). The cells were fixed with 4 % paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55178, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (13)
ab55178 has been referenced in 13 publications.
- Zhang J et al. Long noncoding RNA LINC01287 promotes proliferation and inhibits apoptosis of lung adenocarcinoma cells via the miR-3529-5p/RNASEH2A axis under the competitive endogenous RNA pattern. Environ Toxicol 36:2093-2104 (2021). PubMed: 34254728
- Geusz RJ et al. Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors. Nat Commun 12:6636 (2021). PubMed: 34789735
- Thanan R et al. Opposing Roles of FoxA1 and FoxA3 in Intrahepatic Cholangiocarcinoma Progression. Int J Mol Sci 21:N/A (2020). PubMed: 32151057
- Li H et al. Olfactomedin 4 mediation of prostate stem/progenitor-like cell proliferation and differentiation via MYC. Sci Rep 10:21924 (2020). PubMed: 33318499
- Wang F et al. Gene Expression Profiling Reveals Distinct Molecular Subtypes of Esophageal Squamous Cell Carcinoma in Asian Populations. Neoplasia 21:571-581 (2019). PubMed: 31048097
