Recombinant Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free (ab249749)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10881-14] to FOXA1 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-FOXA1 antibody [EPR10881-14] - BSA and Azide free
See all FOXA1 primary antibodies -
Description
Rabbit monoclonal [EPR10881-14] to FOXA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details
Unsuitable for: IP or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249749 is the carrier-free version of ab173287.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10881-14 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis. -
Tissue specificity
Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3169 Human
- Entrez Gene: 15375 Mouse
- Omim: 602294 Human
- SwissProt: P55317 Human
- SwissProt: P35582 Mouse
- Unigene: 163484 Human
- Unigene: 4578 Mouse
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Alternative names
- forkhead box A1 antibody
- Forkhead box protein A1 antibody
- FOX A1 antibody
see all
Images
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This data was developed using ab173287, the same antibody clone in a different buffer formulation.
IHC-Paraffin-embedded sections of Human prostate hyperplasia. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab173287, the same antibody clone in a different buffer formulation.
Left image: Immunofluorescent staining of SW480 cells using ab173287(1:1000 dilution). 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078). Right image: Immunofluorescent staining of SW480 cells with DAPI. Fixative = 4% paraformaldehyde. -
This data was developed using ab173287, the same antibody clone in a different buffer formulation.
IHC-Paraffin-embedded sections of Mouse colon. 1:1000 dilution of ab173287 used. Counter stain = Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab173287, the same antibody clone in a different buffer formulation.
Left image: Immunofluorescent staining of HepG2 cells using ab173287. 1:200 dilution of Secondary antibody = Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077). Right image: Immunofluorescent staining of HepG2 cells with DAPI. Fixative = 4% paraformaldehyde. -
This data was developed using ab173287, the same antibody clone in a different buffer formulation.
IHC-Paraffin-embedded sections of Human breast carcinoma. 1:1000 dilution of ab173287 used. Counter stain = hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab173287, the same antibody clone in a different buffer formulation.
ab173287 showing -ve staining in Human brain tissue. 1:1000 dilution of ab. Paraffin-embedded sections. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249749 has not yet been referenced specifically in any publications.