Recombinant Anti-FRS2 antibody [EPR14724] (ab183492)
![Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)](https://www.abcam.com/ps/products/183/ab183492/Images/ab183492-214838-anti-frs2-antibody-epr14724-western-blot.jpg "Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14724] to FRS2
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-FRS2 antibody [EPR14724]
See all FRS2 primary antibodies -
Description
Rabbit monoclonal [EPR14724] to FRS2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, HeLa and SH-SY5Y lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14724 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Detects a band of approximately 60-90 kDa (predicted molecular weight: 57 kDa).
|
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| IP |
1/50 - 1/70.
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| Notes |
|---|
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Detects a band of approximately 60-90 kDa (predicted molecular weight: 57 kDa). |
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IP
1/50 - 1/70. |
Target
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Function
Adapter protein that links FGR and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Modulates signaling via SHC1 by competing for a common binding site on NTRK1. -
Tissue specificity
Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis. -
Sequence similarities
Contains 1 IRS-type PTB domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues upon stimulation by NGF.
Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination. -
Cellular localization
Endomembrane system. Cytoplasmic, membrane-bound. - Information by UniProt
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Database links
- Entrez Gene: 10818 Human
- Omim: 607743 Human
- SwissProt: Q8WU20 Human
- Unigene: 593446 Human
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Alternative names
- FGFR signaling adaptor SNT antibody
- FGFR signalling adaptor antibody
- FGFR substrate 2 antibody
see all
Images
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Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)All lanes : Anti-FRS2 antibody [EPR14724] (ab183492) at 1/10000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa -
![Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)](https://www.abcam.com/ps/products/183/ab183492/Images/ab183492-214837-anti-frs2-antibody-epr14724-western-blot.jpg)
Western blot - Anti-FRS2 antibody [EPR14724] (ab183492)Anti-FRS2 antibody [EPR14724] (ab183492) at 1/2000 dilution + SH-SY5Y cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 57 kDa -
![Flow Cytometry (Intracellular) - Anti-FRS2 antibody [EPR14724] (ab183492)](https://www.abcam.com/ps/products/183/ab183492/Images/ab183492-214834-anti-frs2-antibody-epr14724-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-FRS2 antibody [EPR14724] (ab183492)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedSH-SY5Y cells labeling FRS2 with ab183492 at 1/20 dilution (red), compared to aRabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Immunoprecipitation - Anti-FRS2 antibody [EPR14724] (ab183492)](https://www.abcam.com/ps/products/183/ab183492/Images/ab183492-214833-anti-frs2-antibody-epr14724-immunoprecipitation.jpg)
Immunoprecipitation - Anti-FRS2 antibody [EPR14724] (ab183492)Western blot analysis of FRS2 in Human fetal brain lysate immunoprecipitated using ab183492 at 1/50 dilution (Lane 1). Lane 2: TBS instead of Human fetal brain lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-FRS2 antibody [EPR14724] (ab183492)](https://www.abcam.com/ps/products/183/ab183492/Images/ab183492-5-benefits-of-recombinant-antibodies.png)
Anti-FRS2 antibody [EPR14724] (ab183492)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab183492 has been referenced in 5 publications.
- Chan J et al. Systematic pharmacological analysis of agonistic and antagonistic fibroblast growth factor receptor 1 MAbs reveals a similar unique mode of action. J Biol Chem 299:102729 (2023). PubMed: 36410439
- Xie T et al. LHX2 facilitates the progression of nasopharyngeal carcinoma via activation of the FGF1/FGFR axis. Br J Cancer 127:1239-1253 (2022). PubMed: 35864158
- Duan A et al. Long Noncoding RNA XIST Promotes Resistance to Lenvatinib in Hepatocellular Carcinoma Cells via Epigenetic Inhibition of NOD2. J Oncol 2022:4537343 (2022). PubMed: 36304988
- Dadone-Montaudié B et al. Novel Therapeutic Insights in Dedifferentiated Liposarcoma: A Role for FGFR and MDM2 Dual Targeting. Cancers (Basel) 12:N/A (2020). PubMed: 33092134
- Goyal L et al. TAS-120 Overcomes Resistance to ATP-Competitive FGFR Inhibitors in Patients with FGFR2 Fusion-Positive Intrahepatic Cholangiocarcinoma. Cancer Discov 9:1064-1079 (2019). PubMed: 31109923