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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-FXR1 antibody [EPR7932] (ab129089)

Reviews (1) Submit a question References (13)
Western blot - Anti-FXR1 antibody [EPR7932] (ab129089)
  • Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)
  • Flow Cytometry (Intracellular) - Anti-FXR1 antibody [EPR7932] (ab129089)
  • Western blot - Anti-FXR1 antibody [EPR7932] (ab129089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXR1 antibody [EPR7932] (ab129089)
  • Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)
  • OI-RD Scanning - Anti-FXR1 antibody [EPR7932] (ab129089)
  • Anti-FXR1 antibody [EPR7932] (ab129089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7932] to FXR1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

Overview

  • Product name

    Anti-FXR1 antibody [EPR7932]
    See all FXR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7932] to FXR1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Fetal muscle, MCF7, 293T, Hep G2, A549, HeLa, and fetal heart lysates, Human brain tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 70-78 kDa (predicted molecular weight: 70 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/1000 - 1/10000. Detects a band of approximately 70-78 kDa (predicted molecular weight: 70 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for IP.

Target

  • Function

    RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs.

  • Tissue specificity

    Expressed in all tissues examined including heart, brain, kidney and testis.

  • Sequence similarities

    Belongs to the FMR1 family.
    Contains 2 KH domains.

  • Post-translational
    modifications

    Arg-445 is dimethylated, probably to asymmetric dimethylarginine.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Fragile X mental retardation autosomal homolog 1 antibody
    • Fragile X mental retardation syndrome related protein 1 antibody
    • Fragile X mental retardation syndrome-related protein 1 antibody
    • FXR1 antibody
    • FXR1_HUMAN antibody
    • FXR1P antibody
    • hFXR1p antibody
    see all

Images

  • Western blot - Anti-FXR1 antibody [EPR7932] (ab129089)
    Western blot - Anti-FXR1 antibody [EPR7932] (ab129089)
    All lanes : Anti-FXR1 antibody [EPR7932] (ab129089) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : FXR1 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 70-80 kDa why is the actual band size different from the predicted?



    Lanes 1 - 5: Merged signal (red and green). Green - ab129089 observed at 70-80 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

    ab129089 was shown to react with FXR1 in wild-type HeLa cells in Western blot with loss of signal observed in FXR1 knockout cell line ab264017 (knockout cell lysate ab264505). Wild-type HeLa and FXR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab129089 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)
    Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling FXR1 with purified ab129089 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Flow Cytometry (Intracellular) - Anti-FXR1 antibody [EPR7932] (ab129089)
    Flow Cytometry (Intracellular) - Anti-FXR1 antibody [EPR7932] (ab129089)

    ab129089 staining FXR1 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/60. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

    Isoytype control: Rabbit monoclonal IgG (Black) 

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

     

     

  • Western blot - Anti-FXR1 antibody [EPR7932] (ab129089)
    Western blot - Anti-FXR1 antibody [EPR7932] (ab129089)
    All lanes : Anti-FXR1 antibody [EPR7932] (ab129089) at 1/1000 dilution

    Lane 1 : Fetal muscle lysate
    Lane 2 : MCF7 lysate
    Lane 3 : 293T lysate
    Lane 4 : HeLa lysate
    Lane 5 : Fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70-78 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXR1 antibody [EPR7932] (ab129089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FXR1 antibody [EPR7932] (ab129089)

    ab129089, at a dilution of 1/100, staining FXR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)
    Immunocytochemistry/ Immunofluorescence - Anti-FXR1 antibody [EPR7932] (ab129089)
    ab129089, at a dilution of 1/250, staining Hela cells by immunofluorescent staining
  • OI-RD Scanning - Anti-FXR1 antibody [EPR7932] (ab129089)
    OI-RD Scanning - Anti-FXR1 antibody [EPR7932] (ab129089)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-FXR1 antibody [EPR7932] (ab129089)
    Anti-FXR1 antibody [EPR7932] (ab129089)

References (13)

Publishing research using ab129089? Please let us know so that we can cite the reference in this datasheet.

ab129089 has been referenced in 13 publications.

  • Lee YJ  et al. Role of sirtuin-1 (SIRT1) in hypoxic injury in pancreatic ß-cells. J Drug Target 29:88-98 (2021). PubMed: 32749162
  • Sekulovski N  et al. Niclosamide's potential direct targets in ovarian cancer†. Biol Reprod 105:403-412 (2021). PubMed: 33855343
  • Wang H  et al. Protein and Signaling Pathway Responses to rhIL-6 Intervention Before Lobaplatin Treatment in Osteosarcoma Cells. Front Oncol 11:602712 (2021). PubMed: 33791202
  • Smith JA  et al. FXR1 splicing is important for muscle development and biomolecular condensates in muscle cells. J Cell Biol 219:N/A (2020). PubMed: 32328638
  • Yang L & Li T LncRNA TUG1 regulates ApoM to promote atherosclerosis progression through miR-92a/FXR1 axis. J Cell Mol Med 24:8836-8848 (2020). PubMed: 32597038
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-FXR1 antibody [EPR7932]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuro2a cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Neuro2a cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alexandre Magno

Verified customer

Submitted Jun 26 2019

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