Anti-FZD3 antibody (ab217032)
Key features and details
- Rabbit polyclonal to FZD3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FZD3 antibody
See all FZD3 primary antibodies -
Description
Rabbit polyclonal to FZD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human FZD3 aa 150-250 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9NPG1 -
Positive control
- Mouse pancreas lysate; Rat brain, rat kidney and human colon cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100 - 1/1000. Predicted molecular weight: 76 kDa.
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| IHC-P |
1/100 - 1/500.
When using a fluorescent secondary antibody the recommended dilution is 1/50 – 1/200. |
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| ICC/IF |
1/200.
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| Notes |
|---|
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WB
1/100 - 1/1000. Predicted molecular weight: 76 kDa. |
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IHC-P
1/100 - 1/500. When using a fluorescent secondary antibody the recommended dilution is 1/50 – 1/200. |
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ICC/IF
1/200. |
Target
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Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. -
Tissue specificity
Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis. -
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
Domain
Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7976 Human
- Entrez Gene: 14365 Mouse
- Entrez Gene: 266715 Rat
- Omim: 606143 Human
- SwissProt: Q9NPG1 Human
- SwissProt: Q61086 Mouse
- Unigene: 40735 Human
- Unigene: 214687 Mouse
see all -
Alternative names
- Frizzled 3 antibody
- Frizzled 3 seven transmembrane spanning receptor antibody
- Frizzled family receptor 3 antibody
see all
Images
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Western blot - Anti-FZD3 antibody (ab217032)Anti-FZD3 antibody (ab217032) at 1/300 dilution + Mouse pancreas lysate
Secondary
Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/5000 dilution
Predicted band size: 76 kDa12% gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FZD3 antibody (ab217032)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling FZD3 with ab217032 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-FZD3 antibody (ab217032)Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded rat kidney tissue labeling FZD3 with ab217032 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution for 40 minutes at 37°C. DAPI (5 µg/ml) was used to stain the cell nuclei.
Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-FZD3 antibody (ab217032)Immunohistochemical analysis of paraformaldehyde-fixed, paraffin-embedded human colon cancer tissue labeling FZD3 with ab217032 at 1/200 dilution overnight at 4°C, followed by Goat Anti-Rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution for 40 minutes at 37°C. DAPI (5 µg/ml) was used to stain the cell nuclei.
Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab217032 has been referenced in 3 publications.
- Zhu T et al. Stratification of lung adenocarcinoma patients for D-limonene intervention based on the expression signature genes. Food Funct N/A:N/A (2021). PubMed: 34159963
- Liu B et al. Effect of microRNA-27b on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma via FZD7 signaling pathway. Oncol Lett 18:667-673 (2019). PubMed: 31289540
- Yao W et al. Over-expressed LOC101927196 suppressed oxidative stress levels and neuron cell proliferation in a rat model of autism through disrupting the Wnt signaling pathway by targeting FZD3. Cell Signal 62:109328 (2019). PubMed: 31145996