Recombinant Anti-GAB1 antibody [EPR375] (ab133486)
![Western blot - Anti-GAB1 antibody [EPR375] (ab133486)](https://www.abcam.com/ps/products/133/ab133486/Images/ab133486-296570-anti-gab1-antibody-epr375-western-blot.jpg "Western blot - Anti-GAB1 antibody [EPR375] (ab133486)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR375] to GAB1
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GAB1 antibody [EPR375]
See all GAB1 primary antibodies -
Description
Rabbit monoclonal [EPR375] to GAB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human GAB1 aa 500-600. The exact sequence is proprietary.
Database link: Q13480 -
Positive control
- WB: HepG2, K562, 293T, and SH-SY5Y cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR375 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 77 kDa.
|
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| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 77 kDa. |
|
IP
1/10 - 1/100. |
Target
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Function
Probably involved in EGF and insulin receptor signaling. -
Sequence similarities
Belongs to the GAB family.
Contains 1 PH domain. -
Post-translational
modificationsPhosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. - Information by UniProt
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Database links
- Entrez Gene: 2549 Human
- Omim: 604439 Human
- SwissProt: Q13480 Human
- Unigene: 618456 Human
- Unigene: 80720 Human
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Alternative names
- Gab 1 antibody
- GAB1 antibody
- GAB1_HUMAN antibody
see all
Images
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Western blot - Anti-GAB1 antibody [EPR375] (ab133486)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: GAB1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hek293 whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab133486 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133486 was shown to recognize GAB1 in wild-type HAP1 cells as signal was lost at the expected MW in GAB1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GAB1 knockout samples were subjected to SDS-PAGE. Ab133486 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-GAB1 antibody [EPR375] (ab133486)](https://www.abcam.com/ps/products/133/ab133486/Images/ab133486-251282-anti-gab1-antibody-epr375-western-blot.jpg)
Western blot - Anti-GAB1 antibody [EPR375] (ab133486)Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution (purified) + K562 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 77 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-GAB1 antibody [EPR375] (ab133486)](https://www.abcam.com/ps/products/133/ab133486/Images/ab133486-251281-anti-gab1-antibody-epr375-western-blot.jpg)
Western blot - Anti-GAB1 antibody [EPR375] (ab133486)Anti-GAB1 antibody [EPR375] (ab133486) at 1/2000 dilution (purified) + HEK293 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 77 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Western blot - Anti-GAB1 antibody [EPR375] (ab133486)](https://www.abcam.com/ps/products/133/ab133486/Images/ab133486-147139-anti-gab1-antibody-epr375-western-blot.jpg)
Western blot - Anti-GAB1 antibody [EPR375] (ab133486)All lanes : Anti-GAB1 antibody [EPR375] (ab133486) at 1/1000 dilution (unpurified)
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 77 kDa -
![Immunoprecipitation - Anti-GAB1 antibody [EPR375] (ab133486)](https://www.abcam.com/ps/products/133/ab133486/Images/ab133486-251234-anti-gab1-antibody-epr375-immunoprecipitation.jpg)
Immunoprecipitation - Anti-GAB1 antibody [EPR375] (ab133486)ab133486 (purified) at 1/80 immunoprecipitating GAB1 in HEK293 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Anti-GAB1 antibody [EPR375] (ab133486)](https://www.abcam.com/ps/products/133/ab133486/Images/ab133486-7-benefits-of-recombinant-antibodies.png)
Anti-GAB1 antibody [EPR375] (ab133486)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab133486 has been referenced in 5 publications.
- He Q et al. Circ_0061012 contributes to IL-22-induced proliferation, migration and invasion in keratinocytes through miR-194-5p/GAB1 axis in psoriasis. Biosci Rep 41:N/A (2021). PubMed: 33393621
- Visani M et al. miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children. Diagnostics (Basel) 10:N/A (2020). PubMed: 32365560
- Carrie C et al. Exclusive Hyperfractionated Radiation Therapy and Reduced Boost Volume for Standard-Risk Medulloblastoma: Pooled Analysis of the 2 French Multicentric Studies MSFOP98 and MSFOP 2007 and Correlation With Molecular Subgroups. Int J Radiat Oncol Biol Phys 108:1204-1217 (2020). PubMed: 32768563
- Shuangshoti S et al. Simplified Molecular Subtyping of Medulloblastoma for Reduced Cost and Improved Turnaround Time. Appl Immunohistochem Mol Morphol 28:538-543 (2020). PubMed: 31343993
- Kaur K et al. Approach to molecular subgrouping of medulloblastomas: Comparison of NanoString nCounter assay versus combination of immunohistochemistry and fluorescence in-situ hybridization in resource constrained centres. J Neurooncol 143:393-403 (2019). PubMed: 31104222