Recombinant Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)](https://www.abcam.com/ps/products/252/ab252430/Images/ab252430-1-anti-gaba-a-receptor-alpha-1-antibody-epr23539255-immunohistochemistry-mouse-cerebrum.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23539-255] to GABA A Receptor alpha 1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GABA A Receptor alpha 1 antibody [EPR23539-255]
See all GABA A Receptor alpha 1 primary antibodies -
Description
Rabbit monoclonal [EPR23539-255] to GABA A Receptor alpha 1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse and rat cerebrum tissue. WB: Mouse and rat brain, cerebellum and hippocampus tissue lysate. Human brain and cerebellum tissue lysate. IP: Mouse cerebellum tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23539-255 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
|
| WB |
1/1000. Predicted molecular weight: 52 kDa.
|
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IP
1/30. |
|
WB
1/1000. Predicted molecular weight: 52 kDa. |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. -
Involvement in disease
Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood.
Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5) [MIM:611136]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2554 Human
- Entrez Gene: 14394 Mouse
- Entrez Gene: 29705 Rat
- Omim: 137160 Human
- SwissProt: P14867 Human
- SwissProt: P62812 Mouse
- SwissProt: P62813 Rat
- Unigene: 175934 Human
see all -
Alternative names
- ECA4 antibody
- EIEE19 antibody
- EJM antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling GABA A Receptor alpha 1 with ab252430 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse cerebrumis observed (PMID:29467616). The section was incubated with ab252430 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)](https://www.abcam.com/ps/products/252/ab252430/Images/ab252430-388908-anti-gaba-a-receptor-alpha-1-antibody-epr23539255-western-blot-human-brain10-human-cerebellum-10-lysates.jpg)
Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human cerebellum tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)](https://www.abcam.com/ps/products/252/ab252430/Images/ab252430-3-anti-gaba-a-receptor-alpha-1-antibody-epr23539255-immunoprecipitation-mouse-cerebellum-tissue-lysate-10ug.jpg)
Immunoprecipitation - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)GABA A Receptor alpha 1 was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate 10µg with ab252430 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252430 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10µg.
Lane 2: ab252430 IP in mouse cerebellum tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252430 in mouse cerebellum tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 33 seconds.
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![Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)](https://www.abcam.com/ps/products/252/ab252430/Images/ab252430-388910-anti-gaba-a-receptor-alpha-1-antibody-epr23539255-western-blot-mouse-brain10mouse-cerebellum10-mouse-hippocampus10-lysates.jpg)
Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Mouse hippocampus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot of Lane 1-2 was developed using a higher sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).
Exposure time: Lane 1-2: 156 seconds; Lane 3: 3 minutes.
-
![Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)](https://www.abcam.com/ps/products/252/ab252430/Images/ab252430-388911-anti-gaba-a-receptor-alpha-1-antibody-epr23539255-western-blot-rat-brain-10rat-cerebellum-10-rat-hippocampus-10-lysates.jpg)
Western blot - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)All lanes : Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430) at 1/1000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat cerebellum tissue lysate
Lane 3 : Rat hippocampus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 52 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 15542076, 19428632).
Exposure time: 3 minutes.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)](https://www.abcam.com/ps/products/252/ab252430/Images/ab252430-2-anti-gaba-a-receptor-alpha-1-antibody-epr23539255-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling GABA A Receptor alpha 1 with ab252430 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum is observed (PMID:29467616). The section was incubated with ab252430 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)](https://www.abcam.com/ps/products/252/ab252430/Images/ab252430-7-benefits-of-recombinant-antibodies.png)
Anti-GABA A Receptor alpha 1 antibody [EPR23539-255] (ab252430)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab252430 has been referenced in 5 publications.
- Emre C et al. Intranasal delivery of pro-resolving lipid mediators rescues memory and gamma oscillation impairment in AppNL-G-F/NL-G-F mice. Commun Biol 5:245 (2022). PubMed: 35314851
- Zhang X et al. Seizures in PPT1 Knock-In Mice Are Associated with Inflammatory Activation of Microglia. Int J Mol Sci 23:N/A (2022). PubMed: 35628400
- Yuan A et al. Long non-coding RNA Gm37494 alleviates osteoarthritis chondrocyte injury via the microRNA-181a-5p/GABRA1 axis. J Orthop Surg Res 17:304 (2022). PubMed: 35689264
- Wang C et al. Neuropathic Injury-Induced Plasticity of GABAergic System in Peripheral Sensory Ganglia. Front Pharmacol 12:702218 (2021). PubMed: 34385921
- Wang M et al. Schisandrin B exerts hypnotic effects in PCPA-treated rats by increasing hypothalamic 5-HT and ?-aminobutyric acid levels. Exp Ther Med 20:142 (2020). PubMed: 33093880